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| 基于二硒化钼纳米球和杂交链式反应灵敏检测microRNA | |
| 引用本文： | 夏欢,谢星辰,黄克靖. 基于二硒化钼纳米球和杂交链式反应灵敏检测microRNA[J]. 信阳师范学院学报(自然科学版), 2018, 0(2): 258-262 |
| 作者姓名： | 夏欢 谢星辰 黄克靖 |
| 作者单位： | 信阳师范学院化学化工学院 |
| 摘 要： | 利用SiO_2纳米球为模板水热法合成了二硒化钼纳米球,并以此材料为电极基底,电沉积上AuNPs后,进行交联链式反应进行信号放大,并结合过氧化物酶催化H_2O_2+HQ底液产生电化学信号高灵敏检测microRNA-21.结果表明:新方法在1.0×10~(-16)~1.0×10~(-10)mol/L的浓度范围内呈良好的线性关系,检出限为0.16fmol/L(R_(SN)=3).该传感器被成功应用于血清样品分析. |
| 关 键 词： | 二硒化钼 MicroRNA 电化学生物传感器 信号放大 |
Sensitive Detection of MicroRNA Based on MoSe_2 Nanospheres and Hybridization Chain Reaction |
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| Affiliation: | ,College of Chemistry and Chemical Engineering,Xinyang Normal University |
| Abstract: | MoSe_2 spheres were prepared by a simple hydrothermal method using SiO_2 as template and as electrodes material,and applied as a sensing platform after electrodeposition of AuNPs on it. Hybridization chain reaction was used to amplify signal and HRP catalyzing H_2O_2+HQ system was used to produce electrochemical response for detection of microRNA-21. New method showed a good linear relationship ranging from 1.0×10~(-16) to 1.0×10~(-10) mol/L with a detection limit of 0.16 fmol/L( R_(SN)= 3). The proposed biosensor was successfully applied for serum analysis. |
| Keywords: | MoSe_2 microRNA electrochemical biosensor signal amplification |
| 本文献已被 CNKI 等数据库收录！ | |