Sex-related gene and sex identification of Crested IbisNipponia nippon (Ciconiiformes: Threskiornithidae) |
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Authors: | Ming Li Changqing Ding Fuwen Wei Shijie Meng Yongmei Xi Baozhong Lu |
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Institution: | LI Ming, DING Changqing, WEI Fuwen, MENG Shijie, XI Yongmei & LU Baozhong Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; Department of Biology, Northwest University, Xi'an 710060, China; Shaanxi Crested Ibis Conservation Station, Yangxian 723300, China |
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Abstract: | The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual alike bird, it is very difficult to identify the sex from the morphological character. In order to identify the sex easily and select the right individuals for captive breeding and reintroduction, the sex related gene on W chromosome was amplified and the sex of three Crested Ibises were also identified in the present study. The 262bp fragment was also sequenced, and we found that there were 13 different nucleotide sites with 2.25 of transition/transversion based on the comparison with that of the Oriental White Stork. The sequence will also provide a theoretic base for further designing specific primer for sex related gene in the Crested Ibis. |
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Keywords: | Crested Ibis Nipponia nippon sex-related gene sex identification |
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