| 镉对油菜花粉细胞内钙离子浓度的影响 |
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| 引用本文： | 胡金朝.镉对油菜花粉细胞内钙离子浓度的影响[J].西昌学院学报(自然科学版),2010,24(4):1-3. |
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| 作者姓名： | 胡金朝 |
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| 作者单位： | 西昌学院农业科学学院,四川西昌615013 |
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| 基金项目： | 河南省科技厅科技攻关项目，河南省教育厅自然科学基金项目 |
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| 摘 要： | 应用激光扫描共聚焦显微系统(LSCM)和fluo-3 AM荧光探针标记技术,观察了不同浓度Cd2+对油菜花粉细胞内游离Ca2+的影响。发现油菜花粉经水合培养后,胞内Ca2+呈极性分布,萌发沟附近浓度较高,花粉管萌生部位浓度最高;胞外环境中Cd2+可使细胞内游离Ca2+的浓度升高,表明油菜花粉受重金属Cd2+毒害后,可通过调动胞质内游离Ca2+的变化而做出对外界刺激的应答。这一实验结果为探索重金属对植物的毒害机理提供了新的证据。
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| 关 键 词： | 油菜 花粉 Cd^2＋ 低温装载 激光共聚焦显微技术 |
Effect of Cadmium on the Cytosolic Ca2+ Concentration in Brassica campestris L.Pollen |
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| HU Jin-zhao.Effect of Cadmium on the Cytosolic Ca2+ Concentration in Brassica campestris L.Pollen[J].Journal of Xichang College,2010,24(4):1-3. |
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| Authors: | HU Jin-zhao |
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| Institution: | HU Jin-zhao(School of Agricultural Sciences,Xichang College,Xichang,Sichuan 615013) |
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| Abstract: | Effects of different concentrations of Cd^2＋ on the cytosolic Ca^2＋ concentration in Brassica campestris L.pollen were investigated via laser scanning confocal microscopy and the calcium ion-fluorescence indicator fluo-3AM.It is found that the Cytosolic Ca^2＋ distributed polarly in Brassica campestris L.pollen after water culture.The cytosolic Ca^2＋ concentration was higher near the germ furrow and the highest at pollen tube germinating site.The exogenous Cd^2＋ could elevate the intracellular Ca^2＋ concentration,showing that the Brassica campestris L.pollen made the responsion to exoteric stimulative factor by regulating the cytosolic Ca^2＋ concentration.The results provide a new evidence for exploring the toxic mechanism of heavy metal to plant. |
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| Keywords: | Brassica campestris L Pollen Cd^2＋ Low temperature loading Confocal laser scanning microscopy |
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