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| 用酶联免疫吸附试验诊断蓝氏贾第鞭毛虫病免疫价值的研究 | |
| 引用本文： | 陈思礼,陈强,袁媛,李玲,陈思义,廖林贵,SPITHILL Terry,SMOOKER Peter.用酶联免疫吸附试验诊断蓝氏贾第鞭毛虫病免疫价值的研究[J].华中师范大学学报(自然科学版),2004,38(4):497-500. |
| 作者姓名： | 陈思礼 陈强 袁媛 李玲 陈思义 廖林贵 SPITHILL Terry SMOOKER Peter |
| 作者单位： | 1. 中南民族大学,生命科学学院,武汉,430074 2. 华中师范大学,生命科学学院,武汉,430079 3. 湖北省预防医学科学院,武汉,430079 4. 阳新县白沙医院,湖北,阳新,435242 5. 黄石市卫生防疫站,湖北,黄石,435000 6. Department of Molecular Parasitology,Monash University,Clayton,VIC 3168,Australia |
| 基金项目： | Supported by National Natural Science Funds of Australia (ARC large grant (A) 0 0 1 50 1 ) |
| 摘 要： | 用蓝氏贾第鞭毛虫纯培养虫株和超声粉碎可溶性虫体部分作为抗原，采用ELISA方法检测人体感染蓝氏贾第鞭毛虫血清免疫抗体并讨论其寄生虫学诊断价值．检测65份成人贾第虫病血清，其中55份IgG和／或IgA阳性，阳性率84．62％．20份10岁以下儿童贾第虫病人血清无1份阳性．结果提示蓝氏贾第鞭毛虫感染产生的体液免疫反应存在有年龄依赖性，酶联免疫吸附试验用于蓝氏贾第鞭毛虫病免疫诊断具有局限性． |
| 关 键 词： | 循环抗体 蓝氏贾第鞭毛虫 酶联免疫吸附试验 诊断价值 |
Study on the immunodiagnostic value of the enzyme-linked immunosorbent assay in giardiasis |
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| Abstract.Study on the immunodiagnostic value of the enzyme-linked immunosorbent assay in giardiasis[J].Journal of Central China Normal University(Natural Sciences),2004,38(4):497-500. | |
| Authors: | Abstract |
| Abstract: | 85 sera from the residents with Giardia lamblia infections in Hubei province were investigated for the presence of anti-G. lamblia antibodies, using an ELISA with intact as well as sonicated trophozoites as antigen. The sera of twenty nine healthy adults were used as a control group. The humoral immune response was found to be age dependent. In the sera from patients below the age of 10 years, little significant antibodies were found. In adults, circulating IgG antibody was detected in 53.85%(35/65) of the sera and IgA antibody in 30.77%(20/65). Antibodies of either IgG or IgA classes were detected in 84.62% (55/65) of the sera from adults. Humoral IgG and IgA response occurred independently. The results show that the ELISA technique has limited value for diagnosis of giardiasis. |
| Keywords: | circulating antibody Giardia lamblia ELISA diagnostic significance |
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