| 松属林木组织培养不定芽的发生过程──红松胚培养不定芽发生 |
| |
| 引用本文： | 唐翠,詹亚光.松属林木组织培养不定芽的发生过程──红松胚培养不定芽发生[J].哈尔滨师范大学自然科学学报,1997(5). |
| |
| 作者姓名： | 唐翠 詹亚光 |
| |
| 作者单位： | 黑龙江省林业教育学院(唐翠)，东北林业大学(詹亚光) |
| |
| 摘 要： | 红松（PinusKoraiensissieb．etZuce）咸熟胚离体培养，先在分化培养基培养两周，在胚轴上陆续形成不定芽。石蜡制片，观察红松成熟胚不定芽的发生过程．结果表明：不定芽起源于胚轴的皮层细胞，有的是表皮和皮层细胞同时启动。不定芽形成分四个阶段：启动阶段、芽原基形成阶段、鳞叶原基形成阶段和不定芽结构形成阶段．
|
| 关 键 词： | 红松 组织培养 不定芽 发生过程 |
PROCESS OF ADVENTITIOUS BUDS PRODUCED IN VITRO DEVELOPMENT FROM PINUS KORAIENSIS EMBRYOS |
| |
| Tang Cui, Zhan Yaguang.PROCESS OF ADVENTITIOUS BUDS PRODUCED IN VITRO DEVELOPMENT FROM PINUS KORAIENSIS EMBRYOS[J].Natural Science Journal of Harbin Normal University,1997(5). |
| |
| Authors: | Tang Cui Zhan Yaguang |
| |
| Institution: | Tang Cui; Zhan Yaguang(Forestry Education College) (Northeast Forestry University) |
| |
| Abstract: | Cultrure 2week in vitro on medium for differentiation, mature embryos of P. Koraiensis were transferred to the medium without hormone. Adventitious buds produced on epicotyls and hypocotyls. The oranogenesis process of adventitious buds was observed with paraffin section method. The results showed that the adventitious bud arose from cortical cell of hypocotyls,some of them originated from both conical Cells and epidemis cells. The whole process of adventitious buds development in culture could be divided into four stages as follows:(1)strating of cells differentiation (2)bud primordium; (3)shoot apex with needle primordia;(4) adventitious buds. |
| |
| Keywords: | Pinus koraiensis Tissue culture Adventitious buds Process produced |
| 本文献已被 CNKI 等数据库收录！ |
