厌氧-好氧周期循环条件下厌氧磷吸收现象

在厌氧-好氧周期循环反应器中，稳定运行阶段出现规律性的与生物除磷理论相悖的厌氧磷酸盐吸收现象．为此，从厌氧吸收磷、有机物的效果及两者之间的关系、厌氧有机物吸收能量来源等角度，对厌氧吸磷机理进行探讨．采用乙酸钠为主要有机基质，当进水P／COD从2／100增加到4／100，厌氧阶段磷酸盐的去除率一直稳定在50％-70％．在排除化学除磷的情况下，实验结果表明厌氧吸磷与厌氧吸收乙酸盐基质直接相关，而糖原又是有机基质吸收与胞内聚合物储存过程重要的甚至唯一的能量来源．通过对厌氧磷酸盐、有机物、糖原以及混合液中pH值变化的测定分析，初步推测厌氧吸收磷酸盐是当胞内无可利用的磷源时，糖原分解对磷酸盐的需求，并通过敏感于pH值变化的磷载体蛋白，将胞外液相中的无机磷酸盐运输到细胞内部。

Anaerobic Uptake of Phosphate Under Alternation of Anaerobic/Aerobic Condition

Anaerobic uptake of phosphate under alternation of anaerobic/aerobic condition, an exception to the commonly-accepted biological phosphate removal theory, was observed in a sequencing batch reactor (SBR). The mechanism of anaerobic uptake of phosphate was investigated by measuring and analyzing the efficiencies of anaerobic uptake of phosphate and organics, the relation between anaerobic uptake of phosphate and organics, and the energy source of anaerobic organics uptake. With acetate as sole organic substrate, anaerobic phosphate uptake efficiencies remained 50%-70% as the influent P/COD was increased from 2/100 to 4/100. Exclude the main possibility of chemical phosphate removal, the results showed that anaerobic uptake of phosphate was correlated with anaerobic absorption of acetate, and intracellular glycogen was used as the main energy source of anaerobic organics absorption and intracellular polymers storage. Measuring and analyzing the variation of phosphate, organic substrate, intracellular glycogen and pH in the anaerobic phase, a primary explanation was put forward that anaerobic uptake of phosphate was the demand of intracellular glycogen degradation as no intracellular phosphate available, and extracellalur phosphate was transported to intracellular by pH gradient-sensitive phosphate carrier protein.