贻贝多糖的结构修饰及其体外抗肿瘤活性测定

采用三氧化硫吡啶法对厚壳贻贝多糖(MT1)结构进行硫酸化修饰,并对修饰后多糖进行了体外抗肿瘤活性测定。在样品:三氧化硫吡啶=1:6,温度为90℃条件下反应6 h,实验结果表明:硫酸化多糖(MTS)得率为34%,且修饰后硫酸根含量从6.51%提升到32.95%。体外抗肿瘤活性测定结果显示:浓度为8 mg/m L的MT1、MTS对前列腺癌DU-145细胞作用72 h后的抑制率分别为79.35%、85.04%,且IC50为2.04 mg/m L,1.72 mg/m L;而对人肺癌H1299细胞作用72 h后的抑制率分别为:81.28%、85.32%,以及IC50分别为2.46 mg/m L,1.71 mg/m L。结论:在该条件下修饰后的硫酸化贻贝多糖能提高抗肿瘤活性。

Structure Modification and Research on Antitumor Activity of Mussel Polysaccharide

Mussel polysaccharide structure wasmodified bysulphur trioxide pyridine, and antitumor activity of Sulfated polysaccharides was investigated in vitro. The condition of Sulfuric acid esterification experiment was that：the quality proportion of sample and sulphur trioxide pyridine was 1：6, reaction temperature was 90℃and reaction time was 6h.It showed that：the yield of sulfated polysaccharide（MTS） was 34%, and the content of SO42-was improved from 6.51%to 32.95%. Antitumor activity test in vitro showed that：inhibitory effect of MT1, MTS on DU-145 was 79.35%,85.04%, and IC50 was 2.04mg/mL,1.72mg/mL. The inhibitory effectno H1299 was 81.28%, 85.32% and IC50 was 2.46 mg/mL, 1.71mg/mL. Conclusion： Sulfated polysaccharide can improve the antitumor activity.