| 酸苯酚萃取总RNA方法的改进 |
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| 引用本文： | 张琚,冯剑利,赵洪,于自然,陈林锋.酸苯酚萃取总RNA方法的改进[J].南开大学学报,1999,32(2):127-128. |
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| 作者姓名： | 张琚 冯剑利 赵洪 于自然 陈林锋 |
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| 作者单位： | 南开大学分子生物学研究所 |
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| 摘 要： | 目前提取总ＲＮＡ普遍使用的是酸苯酚萃取法（或称硫氰酸胍法）．此法所提取的ＲＮＡ中存有少量基因组ＤＮＡ的污染．为了克服这一缺点，我们对酸苯酚提取ＲＮＡ的方法进行了改进．即先用酸苯酚萃取，然后用蛋白酶Ｋ消化，再次酸苯酚萃取．用改进的方法提取的ＲＮＡ无基因组ＤＮＡ的污染
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| 关 键 词： | 酸苯酚萃取 蛋白酶K消化 |
| 修稿时间： | 1998-04-07 |
THE IMPROVEMENT OF ACID PHENOL EXTRACTION METHOD OF ISOLATING TOTAL RNA |
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| Zhang Ju,Feng Jianli,Zhao Hong,Yu Ziran,Chen Linfeng.THE IMPROVEMENT OF ACID PHENOL EXTRACTION METHOD OF ISOLATING TOTAL RNA[J].Acta Scientiarum Naturalium University Nankaiensis,1999,32(2):127-128. |
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| Authors: | Zhang Ju Feng Jianli Zhao Hong Yu Ziran Chen Linfeng |
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| Abstract: | A method for isolating high pruified total RNA from a sample of flesh or frozen tissues and cells is described. The results of the common acid phenol extraction method show that the RNA isolate was contaminated by a small amount of genomic DNA. In order to overcome this shortcoming, an improved procedure is used by digesting the isolate, which derived from the first acid phenol extraction, with proteinase K and then following an acid phenol extraction once more. |
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| Keywords: | RNA |
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