| 吖啶橙荧光染色法在遗传毒理学中的应用 |
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| 引用本文： | 孔志明.吖啶橙荧光染色法在遗传毒理学中的应用[J].南京大学学报(自然科学版),1990(2). |
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| 作者姓名： | 孔志明 |
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| 作者单位： | 南京大学环境科学系 |
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| 摘 要： | 本文用吖啶橙(Acridine orange)荧光染色法进行了遗传毒理的研究,并取得了较好的效果。实验内容包括:(1)中国仓鼠体外培养细胞的姐妹染色单体交换试验;(2)中国仓鼠活体中姐妹染色单体交换试验;(3)小鼠骨髓嗜多染红细胞微核试验;(4)孕鼠骨髓与胎鼠肝血微核试验。与Giemsa染色法相比,吖啶橙荧光染色法有染色特异性强、色泽鲜艳、重复性强、实验结果可靠等优点.
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| 关 键 词： | 吖啶橙荧光染色 姐妹染色单体交换试验 微核试验 |
APPLICATION ACRIDINE ORANGE FLUORESCENT STAINING METHOD IN GENETIC TOXICOLOGY |
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| Kong Zhiming.APPLICATION ACRIDINE ORANGE FLUORESCENT STAINING METHOD IN GENETIC TOXICOLOGY[J].Journal of Nanjing University: Nat Sci Ed,1990(2). |
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| Authors: | Kong Zhiming |
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| Institution: | Department of Environmental Sciences |
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| Abstract: | Acridine orange fluorescent staining method was used on the studies of genetic toxicology with satisfactory results. In the present paper, following experiments were included: (1) comparison of frequencies on the SCE in various tissuse cultured in vitro at different ages of Chinese hamster, (2) SCE test in vivo of Chinese hamster, (3) micronucleus test of polychromatic erythrocytes in bone marrow of mice, (4) microunucleus test for polychromatic erythrocytes in bone marrow of pregnant mice and in the blood of the fetal liver. The results showed that compared with Giemsa staining, this method has a brighter colour, better reproducibility and is more suitable for micronucleus and therefore more reliable. |
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| Keywords: | acridine orange fluorescent staining SCE (sister chromatid exchange) micronuncleus test |
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