开口箭对小鼠5-羟色胺分泌的影响

目的:采用灌胃、石蜡切片、免疫组化染色和ELISA等方法,探讨开口箭对小鼠5-羟色胺(5-HT)分泌的影响.方法选取20²2g健康昆明小鼠20只,均分成A,B,C,D 4组,分别以蒸馏水和0.25、0.50、1.00mg/mL的开口箭灌胃,0.20mL/次/d,连续灌胃7d.第8d对全部小鼠摘眼球取血,ELISA检测血清5-HT质量浓度;取血后立即处死小鼠,取十二指肠,4%甲醛固定,石蜡切片,免疫组化染色,镜下统计5-HT阳性细胞数量.结果与A组相比,B,C,D组小鼠血液5-HT质量浓度逐渐降低,十二指肠肠壁组织中5-HT阳性细胞数也明显减少.结论开口箭会下调小鼠5-HT的分泌.

Effects of Tupistra chinensis on 5-hydroxytryptamine secretion in mice

Objective:By the methods of gavage,paraffin section,immunohistochemical staining,and ELISA,the effects of Tupistra chinensis on 5-hydroxytryptamine secretion was studied in mice.Methods:Twenty healthy Kunming mice weighing 20-22g were selected and divided into A,B,C and D groups on average.In a serial 7days,drenched respectively distilled water to A group mice and 0.25,0.50,1.00mg/ml Tupistra chinensis diluted solution to B,C and D group mice,with the dose of 0.2ml/t/d.At 8th day,the blood of all the mice was collected by the method of eyeball picking and the content of 5-hydroxytryptamine in blood serum was analysis by the method of ELISA,and meanwhile,all of mice were killed rapidly,the duodenums were harvested,fixed in 4%formaldehyde,made paraffin sections,stained by immunohistochemical methods,and5-hydroxytryptamine positive cells were observed and count under light microscope.Results:From A to D group mice,the content of 5-hydroxytryptamine in blood serum were decreased gradually,and also the quantities of 5-hydroxytryptamine positive cells in duodenums were less and less.Conclusion:Tupistra chinensis can downregulate secretion of 5-hydroxytryptamine in mice.