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| Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells | |
| 引用本文： | ZHU Xiaodong,YAO Feng,TIEN Po. Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells[J]. 自然科学进展(英文版), 2003, 13(2): 114-118 |
| 作者姓名： | ZHU Xiaodong YAO Feng TIEN Po |
| 作者单位： | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China,Laboratory of Tissue Repair and Gene Transfer, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
| 基金项目： | Supported by the Major State Basic Research Development Program of China (G1999075602) |
| 摘 要： | A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore, the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested. |
| 关 键 词： | tetracycline inducible expression system enhanced green fluorescent protein hepatitis B virus tra |
Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells |
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| ZHU Xiaodong,YAO Feng,TIEN Po. Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells[J]. Progress in Natural Science, 2003, 13(2): 114-118 | |
| Authors: | ZHU Xiaodong YAO Feng TIEN Po |
| Affiliation: | 1. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China 2. Laboratory of Tissue Repair and Gene Transfer, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA |
| Abstract: | A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore, the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested. |
| Keywords: | tetracycline inducible expression system enhanced green fluorescent protein hepatitis B virus tra |
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