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| Effects of Cu2+ and Ph on osteoclastic bone resorption in vitro | |
| 引用本文： | ZHANG Jinchao,HUANG Jian,XU Shanjin,WANG Kui,YU Shifeng. Effects of Cu2+ and Ph on osteoclastic bone resorption in vitro[J]. 自然科学进展(英文版), 2003, 13(4): 266-270 |
| 作者姓名： | ZHANG Jinchao HUANG Jian XU Shanjin WANG Kui YU Shifeng |
| 作者单位： | Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China,Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China,Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China,Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China,Department of Oral Pathology, Stomatological Hospital, Peking University, Beijing 100081, China |
| 基金项目： | Supported by the National Natural Science Foundation of China (Grant No.20031010) |
| 摘 要： | The effects of Cu2+ and pH on osteoclastic bone resorption lacunae are studied by culturing Japanese white rabbit osteoclasts on bone slices. The number and surface area of bone resorption lacunae are measured by photomicrography and image analysis. Concentration of calcium ion in the supernatant is measured by atomic absorption spectrometry to evaluate the activity of osteoclasts. The morphology of the lacunae is observed under a scanning electron microscope. The results indicate that Cu2+ at concentration of 1.00×10-6 mol/L and 1.00×10-7 mol/L inhibits osteoclastic activity and causes a dose-dependent reduction in the number and surface area of the lacunae. While the number of lanunae is increased and osteoclastic bone resorbing function is significantly improved at Cu2+ concentration of 1.00×10-8 mol/L. It is suggested that the effect of Cu2+ on osteoclastic bone resorption depends on Cu2+ concentration. pH had no significant effect on osteoclastic function in the near neutral range. |
| 关 键 词： | Cu2+ pH osteoclasts bone resorption |
Effects of Cu2+ and Ph on osteoclastic bone resorption in vitro |
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| Zhang Jinchao,HUANG Jian,Xu Shanjin,WANG Kui,YU Shifeng. Effects of Cu2+ and Ph on osteoclastic bone resorption in vitro[J]. Progress in Natural Science, 2003, 13(4): 266-270 | |
| Authors: | Zhang Jinchao HUANG Jian Xu Shanjin WANG Kui YU Shifeng |
| Affiliation: | 1. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China 2. Department of Oral Pathology, Stomatological Hospital, Peking University, Beijing 100081, China |
| Abstract: | The effects of Cu2+ and pH on osteoclastic bone resorption lacunae are studied by culturing Japanese white rabbit osteoclasts on bone slices. The number and surface area of bone resorption lacunae are measured by photomicrography and image analysis. Concentration of calcium ion in the supernatant is measured by atomic absorption spectrometry to evaluate the activity of osteoclasts. The morphology of the lacunae is observed under a scanning electron microscope. The results indicate that Cu2+ at concentration of 1.00×10-6 mol/L and 1.00×10-7 mol/L inhibits osteoclastic activity and causes a dose-dependent reduction in the number and surface area of the lacunae. While the number of lanunae is increased and osteoclastic bone resorbing function is significantly improved at Cu2+ concentration of 1.00×10-8 mol/L. It is suggested that the effect of Cu2+ on osteoclastic bone resorption depends on Cu2+ concentration. pH had no significant effect on osteoclastic function in the near neutral range. |
| Keywords: | Cu2+ pH osteoclasts bone resorption |
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