| Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts |
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| 引用本文： | Zhang Dawei,Zhang Jinchao,Chen Yao,Yang Mengsu and Yao Xinsheng. Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts[J]. 自然科学进展, 2007, 17(5): 618-623 |
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| 作者姓名： | Zhang Dawei Zhang Jinchao Chen Yao Yang Mengsu and Yao Xinsheng |
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| 作者单位： | Department of Natural Products Chemistry，Shenyang Pharmaceutical University，Shenyang 110016，China; Shenzhen Research Institute of City University of Hong Kong，Shenzhen 518057，China; Department of Biology and Chemistry，City University of Hong Kong，China; Department of Chemistry，College of Chemistry & Environmental Science，Hebei University，Baoding 071002，China; Key Laboratory for Research and Development of Traditional Chinese Medicine and Natural Medicine at Shenzhen，Shenzhen 518057，China |
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| 基金项目： | Supported by National Natural Science Foundation of China (Grant No. 30418007) |
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| 摘 要： | The effects of La3+ and Gd3+ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method， measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3+ and Gd3+ inhibited the proliferation of ROB cells at all concentrations (1×10-5, 1×10-6, 1×10-7, 1×10-8, 1×10-9 mol?L-1). La3+ at concentration of 1×10-5 mol?L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd3+ played a negative role in the alkaline phosphatase activity. La3+ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd3+ promoted the adipogenic transdifferentiation of ROB cells at 1×10-8 and 1×10-9 mol?L-1. These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species
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| 关 键 词： | lanthanum， gadolinium， osteoblast-like cells， proliferation， differentiation， transdifferentiation |
Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts |
| |
| Zhang Dawei,Zhang Jinchao,Chen Yao,Yang Mengsu and Yao Xinsheng. Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts[J]. , 2007, 17(5): 618-623 |
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| Authors: | Zhang Dawei Zhang Jinchao Chen Yao Yang Mengsu Yao Xinsheng |
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| Affiliation: | 1. Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China; 3. Department of Biology and Chemistry, City University of Hong Kong, China; 4. Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China; 5. Key Laboratory for Research and Development of Traditional Chinese Medicine and Natural Medicine at Shenzhen, Shenzhen 518057, China |
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| Abstract: | |
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| Keywords: | lanthanum gadolinium osteoblast-like cells proliferation differentiation transdifferentiation. |
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