两种新型光敏剂的光谱特性研究

研究两种新型的光敏剂癌光啉（PsD-007）和血啉甲醚（HMME）分别在含10%人血清生理盐水和纯生理盐水中的吸收光谱、荧光激发和发射光谱，并与血卟啉衍生物（HpD）进行实验比较。实验结果表明：三种光敏剂在人血清环境中的吸收峰都位于紫外400nm处，但与生理盐水环境相比索瑞峰发生了10nm的红移。光敏剂的荧光激发光谱与它的吸收光谱十分相似。当用413.1nm的紫光激发时，三种光敏剂在人血清环境中的荧光发射光谱在红光区都只有一个位于620nm处的荧光峰，其中HMME的荧光激发效率最高，HpD次之，PsD-007最低。这些结论对于这两种新型光敏剂在光动力诊断和治疗恶性肿瘤中的临床应用具有重要的指导意义。

Study of the Spectrum Properties for Two Kinds of New Photosensitizer

The absorption spectra, fluorescence excitation and emission spectra of new photosensitzer PsD 007 and HMME in the physiological saline with 10 percent serum and in the physiological saline have obtained, respectively.Furthermore,the results are also analyzed and compared with the traditional photosensitizer HpD.Experimental results show that the maximum absorption peaks for three kinds of photosensitizer in the physiological saline with 10 percent serum appear at 400 nm in the soret region, but the peaks are shifted to 390 nm in the physiological saline. The fluorescence excitation spectrum closely resembles the absorption spectrum. It should be taken into account that the former spectrum is recorded with higher resolution than the latter due to different experimental condition. There is only one fluorescence peak appear at 620 nm in fluorescence emission spectra for three kinds of photosensitizer when excited by 413 1 nm. The fluorescence excited efficiency (photons emitted per photo absorbed) of HMME is the highest, and the PsD 007 is lowest among three kinds of photosensitizer. The resulfs presented here will be great significance for the clinical application of photodynamic diagnosis (PDD) and photodynamic therapy (PDT) tumor.