两层 CPO 修饰电极的制备及其应用

电化学方法制备聚L－赖氨酸膜（PLL）修饰的玻碳电极上固定氯过氧化物酶，利用循环伏安法研究了修饰电极的电化学行为．修饰一层和两层的CPO修饰电极上均观察到CPO直接可逆的电子传递，其中，修饰两层CPO的电极（CPO／PLL）2／GC的氧化还原峰电流较为明显；氧气饱和的缓冲溶液中的循环伏安曲线显示，（CPO／PLL）2／GC电极上氧的还原峰电流显著增大，且还原峰电位相对于修饰一层CPO的电极正移了150mV，表明修饰两层CPO的电极对氧还原具有更好的电催化效应．（CPO／PLL）2／GC修饰电极应用于原位产生过氧化氢催化苯甲硫醚的反应，得到了目标产物苯甲亚砜，产物的量随电解催化反应时间的增加而增大．实验结果为开发环保、绿色的生物酶催化有机合成反应提供了新思路．

The preparation of two-layer chloroperoxidase modified electrode and its application

Chloroperoxidase(CPO) was immobilized onto the glassy carbon electrode modified by the electrochemical deposited Poly L——lysine(PLL) membrane.The electrochemical behaviors of CPO modified electrodes were investigated by cyclic voltammetry.The direct reversible electron transfer was observed both on the monolayer and two-layer CPO modified electrodes,but the redox peak current on the two-layer CPO modified electrode was more obvious.The CVs results of(CPO/PLL)2/GC modified electrode in oxygen-saturated PBS phosphate buffer solution showed that oxygen reduction peak current on the two-layer enzyme modified electrode increased significantly and reduction peak potential had a positive shift about 150 mV comparing to monolayer CPO modified electrode.The results showed that the electrocatalytic activity of two-layer CPO modified electrode for oxygen reduction was better than others.(CPO/PLL)2/GC electrode had been used for the catalytic oxidation of thioanisole in which H2O2 was generated in situ electrochemically.Through the initial catalytic reduction of oxygen to H2O2 at the(CPO/PLL)2/GC electrode,the CPO enzyme was activated for the catalytic oxidation of thioanisole forming methyl-phenylsulfoxide.The target product(methyl-phenylsulfoxide) quantity increased as the electrolysis time increased.This study provides a new way for environment-friendly and green organic synthesis reaction catalyzed by the biological enzyme.