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| 夫西地酸产生菌菌落形态与代谢特征的研究 | |
| 引用本文： | 邓 昕,蓝 鸿,董晓景,李继安,陈代杰. 夫西地酸产生菌菌落形态与代谢特征的研究[J]. 上海师范大学学报(自然科学版), 2012, 41(3): 312-318 |
| 作者姓名： | 邓 昕 蓝 鸿 董晓景 李继安 陈代杰 |
| 作者单位： | 1. 上海师范大学生命与环境科学学院,上海,200234 2. 上海医药工业研究院创新药物与制药工艺国家重点实验室,上海,200040 3. 中国医药工业研究总院,上海,200040 |
| 基金项目： | 国家自然科学基金，国家自然科学基金，上海市青年科技启明星计划(B类)，国家“重大新药创制”科技重大专项 |
| 摘 要： | 通过对梭链孢脂球菌SIPI—A．3201进行紫外诱变处理，挑选出3种比较典型的单菌落．摇瓶发酵考察它们的生长代谢特性，检测茵体浓度、糖氮代谢、pH变化以及夫西地酸生物合成量等几项指标．并且对菌株Ⅰ和菌株Ⅲ发酵过程中的菌丝形态变化进行了研究．结果表明：与菌株Ⅰ和菌株Ⅱ相比，菌株Ⅲ草帽型的单菌落产抗水平最高，达到了约480×10-6g／mL． |
| 关 键 词： | 夫西地酸 茵落形态 代谢特性 |
Study on the relationship between colonial morphology of Fusidium coccineum and metabolic characteristics |
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| DENG Xin , LAN Hong , DONG Xiao-jing , LI Ji-an , CHEN Dai-jie. Study on the relationship between colonial morphology of Fusidium coccineum and metabolic characteristics[J]. Journal of Shanghai Normal University(Natural Sciences), 2012, 41(3): 312-318 | |
| Authors: | DENG Xin LAN Hong DONG Xiao-jing LI Ji-an CHEN Dai-jie |
| Affiliation: | 1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China; 2.Shanghai Institute of Pharmaceutical Industry,State Key Lab of New Drug and Pharmaceutical Process,Shanghai lnstitute of Pharmaceutical lndustry,Shanghai 200040,China; 3.China Pharmaceutical Industry Research Institute,Shanghai 200040,China) |
| Abstract: | Three mutants having typical colonies with different morphology of Fusidium coccineum SIPI-A.3201 named Ⅰ,Ⅱ and Ⅲ in this paper were obtained in the process of strain improvement by UV mutation.Metabolic characteristics of them,such as cell growth,changes of pH,nitrogen and sugar,and the biosynthesis of fusidic acid,were measured in the course of fermentation in shaking-flask.The result showed that the mutant with typical morphology of colony Ⅲ biosynthezised highest fusidic acid,up to 480×10-6g/mL. |
| Keywords: | Fusidic acid colonial morphology metabolic characteristics |
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