白芍总苷对NAFLD大鼠肝脏NF—kB／p65蛋白表达及羟脯氨酸含量的影响

目的：观察白芍总苷（TGP）对高脂饮食诱导的非酒精性脂肪肝NAFLD大鼠肝组织核转录因子-κB（NF-κB）和羟脯氨酸（Hyp）及血清血清谷草转氨酶（AsT）、谷丙转氨酶（ALT）、还原型谷胱甘肽（GSH）和白介素1β（IL-1β）、肿瘤坏死因子α（TNF—α）和白介素-6（IL-6）水平的影响．方法：结果：TGP200mg·kg-1组能明显降低NAFLD模型组大鼠血清ALT和AST水平和提高GSH含量（P〈0．01）、能明显抑制肝组织中k的阳性表达及Hyp的生成（P〈0．01）、使血清IL-1B、TNF-a和IL-6水平明显降低（P〈0．01）、使肝脏的病理学改变明显减轻．TGP100mg·kg-1对上述指标无明显改变．结论：TGP能明显改善NAFLD大鼠的肝功能及病理性变化，其机制可能是通过下调NF-κB／p65的表达、降低血清IL-1β、TNF-n和IL-6水平、抑制肝脏Hyp的产生，从而对NAFLD大鼠发挥治疗作用．

Influence of Total Glucosides of Paeony on NF-kB/p65 Protein Expression and Hydroxyproine Content in Nonalcoholic Fatty Liver Disease Rat Liver

Objective：To investigate the influence of total glucosides of paeony on NF - kB/p65 pro- tein expression , the content of hydroxyproine （Typ）.serum alanine aminotransferase（ALT）, aspartate aminotransferase （AST） , glutathione （GSH） and Interleukin 1β（IL-1β）, tumor necrosis factor alpha （TNF- α） and interleukin -6 （IL-6） in nonalcoholic fatty liver disease rat induced by high fat diet. Meth- ods. the NAFLD rat model was established by high fat diet. All rats were randomly divided into 4 groups, normal group, model group, TGP （100 mg · kg-1 .200 mg · kg-1）group, at the end of four weeks, the rats were killed by 3% barbiturates, taking blood from heart, the level of serum AST and ALT was determinated by Henry mothed, the content of GSH was determinated by colorimetric meth- od,the level of IL-1β.TNF-αand IL- 6 was tested by ELISA, extracting liver for HE staining to observe hepatocyte pathological changes, NF-kB/p65 protein expression in the liver tissue was detected by im- munohistochemistry, the content of Hyp was detected by chloroform -T method. Results： TGP（200 nag · kg-1）could significantly reduce the level of serum AST and ALT , and improve the level of GSH in NAFLD rats（P〈0.01）, TGP（200 mg · kg-1）could significantly inhibt the expression of NF-kB/p65 and the formation of Hyp in liver（P〈0.01）, and reduc the contents of serum IL-1β.TNF-α and IL-6（P d0.01）, TGP（200 mg · kg-1）could significantly improve the histopathological changes of liver. TGP （100 mg · kg-1） had no significant effect on the above indicators. Conclusion. TGP（200 mg · kg-1） could significantly improve the liver function and histopathological changes in NAFLD rats, the mecha- nism might be related to down regulating the expression of NF-kB/p65,reducing the level of serum IL- 1β.TNF-α and IL-6,inhinting the formation of Hyp in liver, and to play a role in the treatment of NAFLD rats.