| 啤酒酵母（Saccharomyces cerevisiae）M－05变株合成谷胱甘肽的研究Ⅰ．菌种的选育 |
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| 引用本文： | 施碧红,黄建忠,施巧琴,吴松刚.啤酒酵母（Saccharomyces cerevisiae）M－05变株合成谷胱甘肽的研究Ⅰ．菌种的选育[J].福建师范大学学报(自然科学版),1996(4). |
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| 作者姓名： | 施碧红 黄建忠 施巧琴 吴松刚 |
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| 作者单位： | 福州大学生物工程所 |
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| 摘 要： | 从１２种５５株酵母菌株中筛选出１株相对富含谷胱甘肽（ＧＳＨ）的菌株──啤酒酵母Ｓ─１２，经紫外线及硫酸二乙酯等复合诱变处理，以甲硫氨酸缺陷型（Ｍｅｔ－）为筛选模型，获得１株高产谷胱甘肽变株Ｍ—０５，其干细胞每ｇ含有ＧＳＨ１４．４３ｍｇ，较出发菌株提高了６８．４％．
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| 关 键 词： | 啤酒酵母，谷胱甘肽，甲硫氨酸缺陷型，选育 |
Study on the Synthesis of Glutathione by Saccharomyces cerevisiae Mutant M-05 I.Breeding of High Glutathione Producing Strain |
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| Shi Bihong,Huang Jianzhong,Shi Qiaoqing,Wu Songgang.Study on the Synthesis of Glutathione by Saccharomyces cerevisiae Mutant M-05 I.Breeding of High Glutathione Producing Strain[J].Journal of Fujian Teachers University(Natural Science),1996(4). |
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| Authors: | Shi Bihong Huang Jianzhong Shi Qiaoqing Wu Songgang |
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| Institution: | Institute Of Microbial Engineering |
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| Abstract: | Saccharomyces cerevisiae strain S-12 which riched in glutathione was selected from 55 yeast Strains.Seventy-six mutants of nutrtion-defected were obtained from 2600 colonies picked from strain S-12 through mutation breeding UV-DES by chance.There were 5 mutants of methionine defected among those nutrition-defected mutants.Compared with the Original strain S-12 the intracelluar content Of glthethione of the mutant M-05 reached 14.4mg/g cells and increased by 68.4% than the original strain.Methionine and Selenium could promote mutant M-05 producing glutathione. |
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| Keywords: | Saccharomyces cerevisiae glutathione methionine mutation |
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