高效褐藻胶降解菌的筛选与产酶条件优化

以海藻酸钠为唯一碳源,从天然腐烂海带中筛选得到一株高效褐藻胶降解菌株53#,经形态学观察和16S r RNA鉴定,确定为类芽孢杆菌Paenibacillus agaridevorans。采用正交试验方法,以p H、温度、Na Cl浓度和海藻酸钠初始浓度为影响因素,对该菌株的产酶条件进行优化,得到53#菌的最佳产酶条件:p H=8,25℃,Na Cl浓度15 g/L,海藻酸钠初始浓度15 g/L。在最佳产酶条件下,褐藻胶裂解酶最大酶活可达390.53±17.32U/m L。筛选得到的类芽孢杆菌Paenibacillus agaridevorans 53#具有易于培养、产酶速度快和酶活力高等优点,能够实现褐藻胶的高效糖化,在褐藻生产生物乙醇领域具有潜在利用价值。

Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-Producing Conditions

53#, a strain of efficient alginate degrading bacteria, is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified asPaenibacillusagaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53# is identified at pH=8, T=25oC, NaCl 15 g/L, and sodium alginate 15 g/L by orthogonal experiment and analysis, and the highest enzyme activity is 390.53±17.32 U/mL. Strain 53# has the advantages such as being cultivated easily, producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.