| 新亚甲蓝修饰玻碳电极测定过氧化氢 |
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| 引用本文： | 张文保,凡素华,武海,崔玉民.新亚甲蓝修饰玻碳电极测定过氧化氢[J].阜阳师范学院学报(自然科学版),2010,27(2):47-50,54. |
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| 作者姓名： | 张文保 凡素华 武海 崔玉民 |
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| 作者单位： | 1. 阜阳师范学院化学化工学院,安徽,阜阳,236041;安徽环境污染物降解与监测省级实验室,安徽,阜阳,236041
2. 阜阳师范学院化学化工学院,安徽,阜阳,236041 |
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| 基金项目： | 安徽高校省级自然科学重点项目，阜阳师范学院质量工程项目，阜阳师范学院青年项目 |
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| 摘 要： | 利用新亚甲蓝(NMB)为中间媒介体,以对苯二甲醛为桥联试剂将其与辣根过氧化物酶(HRP)修饰于玻碳电极表面,制备成过氧化氢电化学传感器。实验表明,NMB作为中间介体与对苯二甲醛键合后能有效地传输电子,可求得电子转移系数(α)为0.84,表观反应速率常数Ks为1.09 s-1。该传感器能快速地对过氧化氢响应,线性范围为0.0~75.0mol L-1,检出限低至0.278 mol.L-1。该方法用于检测过氧化氢简单快速而又灵敏,性能稳定。
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| 关 键 词： | 过氧化氢 电化学传感器 新亚甲蓝 |
Electrochemical determination of hydrogen peroxide with new methylene blue modified glassy carbon electrode |
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| ZHANG Wen-bao,FAN Su-hua,WU Hai,CUI Yu-min.Electrochemical determination of hydrogen peroxide with new methylene blue modified glassy carbon electrode[J].Journal of Fuyang Teachers College:Natural Science,2010,27(2):47-50,54. |
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| Authors: | ZHANG Wen-bao FAN Su-hua WU Hai CUI Yu-min |
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| Abstract: | An electrochemical sensor of hydrogen peroxide based on new methylene blue(NMB) as mediator has been developed by modifying NMB and horseradish peroxidase(HRP) on the surface of glassy carbon electrode with ρ-phthalaldehyde as bridging reagent.The experiments show that NMB as mediator coimmobilized with ρ-phthalaldehyde can effectively transfer electrons.The electron transfer coefficient(α) of 0.84 and apparent reaction rate constant(Ks) of 1.09 s-1 are determined.The sensor can quickly respond to hydrogen peroxide with the linear range of 0.0 ~75.0 mol L-1 and the detection limit of 0.278 mol L-1.The method for the detection of hydrogen peroxide is simple,quick and sensitive and has stable performance. |
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| Keywords: | hydrogen peroxide electrochemical sensor new methylene blue |
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