大鼠脊髓楔型半切空洞模型的建立及评估

摘要: 目的建立一种新型大鼠脊髓半切模型，为临床脊髓空洞损伤研究提供适宜的动物模型。方法25 只SD 大鼠随机分为脊髓楔型块状半切组( A 组，n = 10) 、假手术组( B 组，n = 10) 和正常对照组( C 组，n = 5) 。术后采用 BBB 运动功能评分、斜板试验、HE 染色和核磁共振( MRI) 检测综合评价脊髓半切模型的稳定性。结果术后A 组 大鼠伤侧后肢BBB 运动功能评分及斜板维持最大角度值显著低于B 组和C 组( P ＜ 0. 05) ，而这些指标在B 组和C 组之间没有明显差异; 术后28 d HE 和MRI 检测显示脊髓损伤区( A 组) 灰质和白质发生明显的形态学改变。结论 通过该方法建立的脊髓半切模型稳定、可靠，适于脊髓空洞性损伤的研究。

Establishment and Evaluation of a Wedged-block-hemisected Injury Model in Rats Spinal Cord

Objective Establishment a novel hemisected spinal cord injury model with rats to supply model for clinical research.Method 25 SD rats were randomly divided into wedged-block hemisected group （A group,n =10）,sham operation group （B group,n =10） and control group （C group,n =5）.After operation,the stability of the model was comprehensive evaluated by grading the scores of Basso-Beattie-Bresnahan （BBB）,swash plate test,HE staining and MRI assay.Result After operation,the mean scores of BBB in injured hind limb and maximum angle of swash plate in group A were significantly lower than group C and B （P ＜ 0.05）,but these parameters without different between B group and C group; 28 days after operation,HE staining and MRI assay shows a significant morphological changes in spinal cord tissue of group A; Conclusion The results suggested that the rat model established by this method with high quality of stability and reliability which suitable to evaluate the spinal cord injury research.