| 蕨麻水溶性多糖的提取及其单糖组分的分析 |
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| 引用本文： | 刘春兰,雷美康,邓义红,李转秀,曾明.蕨麻水溶性多糖的提取及其单糖组分的分析[J].中央民族大学学报(自然科学版),2007,16(1):16-18. |
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| 作者姓名： | 刘春兰 雷美康 邓义红 李转秀 曾明 |
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| 作者单位： | 中央民族大学,生命与环境科学学院,北京,100081 |
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| 摘 要： | 本文采用热水提取、乙醇沉淀、a-淀粉酶法脱淀粉、链酶蛋白酶和Sevag法联合除蛋白,得到蕨麻多糖.对提取的蕨麻多糖进行了总糖含量及糖醛酸含量的测定.粗多糖(P1)总糖含量为41.8%,糖醛酸含量为11.1%.脱淀粉脱蛋白多糖(P2)总糖含量为39.4%,糖醛酸含量为35.9%.经纸层析和高效液相色谱分析,表明该多糖主要由阿拉伯糖、鼠李糖、葡萄糖、半乳糖、半乳糖醛酸组成,说明蕨麻多糖为酸性杂多糖.
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| 关 键 词： | 蕨麻 多糖 分离纯化 组成 |
| 文章编号： | 1005-8036（2007）01-0016-03 |
| 修稿时间： | 2006-09-04 |
Extraction and Analysis of Water Soluble Polysaccharide of Potentilla anserine L. |
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| LIU Chun-lan,LEI Mei-kang,DENG Yi-hong,LI Zhuan-xiu,ZENG Ming.Extraction and Analysis of Water Soluble Polysaccharide of Potentilla anserine L.[J].Journal of The Central University for Nationalities(Natural Sciences Edition),2007,16(1):16-18. |
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| Authors: | LIU Chun-lan LEI Mei-kang DENG Yi-hong LI Zhuan-xiu ZENG Ming |
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| Institution: | College of Life and Environmental Science, Central University.fi |
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| Abstract: | The crude polysaccharide was extracted from Potentilla anserina L.with hot water,precipitated by ethanol,and was isolated and purified.The total sugar of P1,P2 was 41.8 %,39.4% respectively,and the content of glucuronic acid was 11.1%,35.9% respectively.P2 was a glycoprotein with 15.2 % of protein.The crude polysaccharide was analysised by PC and HPLC,They mainly consiste of Ara,Rha,Gal,Glc,GalA. |
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| Keywords: | Potentilla anserine L polysaccharide Isolition purification composition |
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