米非司酮对大鼠在位及异位子宫内膜COX-2表达的影响

目的:探讨米非司酮对大鼠在位及异位子宫内膜环氧合酶-2(COX-2)表达的影响。方法:子宫内膜异位症(endom etriosis,EMS)大鼠随机分为正常对照组、低剂量和高剂量米非司酮组,灌胃4周后,分别取子宫内膜组织用免疫组化法测COX-2的表达。结果:1)不同实验组间在位内膜COX-2的表达强度均有明显差异,米非司酮高剂量组低于低剂量组(P<0.01)和对照组(P<0.01),低剂量组在位内膜COX-2的表达强度也明显低于正常对照组(P<0.01)。2)米非司酮高剂量组异位内膜COX-2的表达强度低于正常对照组(P<0.01)和低剂量组(P<0.01),而低剂量组异位内膜COX-2的表达强度和对照组比较差异无显著性。3)米非司酮高剂量、低剂量组异位内膜COX-2的表达强度均明显高于在位内膜(P<0.01),而对照组在位、异位内膜COX-2的表达强度差别无显著性。结论:米非司酮治疗EMS可能与降低COX-2的表达有关。

Effect of mifepristone on the expression of COX- 2 in rat eutopic and ectopic endometrium

Aim: To explore the effect of mifepristone on the expression of COX-2 in rat eutopic and ectopic endometrium.Methods:Endometriotic rats were randomly divided into control group and mifepristone group(including low dose and high dose groups).4 weeks after gastrogavage with tuwen-80 only(control group) or tuwen-80 plus mifepristone 0.62 mg/d(low dose group) or 1.24 mg/d(high dose group),all rats were killed and tissues of ectopic and eutopic endometrium were removed for detecting COX-2 expression via immunohistochemistry(S-P method). Results:1) In the eutopic endometrium,the expression intensity of COX-2 in the high dose group was lower than that in the low dose group and the control group(P<0.01).The expression intensity of COX-2 in the low dose group was lower than that in the control group(P<0.01).2) In the ectopic endometrium,the expression intensity of COX-2 in the high dose group was lower than that in the low dose group(P<0.01)and the control group(P<0.01),but it was no markedly difference between the low dose group and the control group.3)In both high dose and low dose groups,the expression intensity of COX-2 in the ectopic endometrium was higher than that in the eutopic endometrium (P<0.01),but in the control group,it was no significantly difference in the expression intensity of COX-2 between the ectopic endometrium and the eutopic endometrium.Conclusion:The treament effect of mifepristone on EMS might be related with decreased expression of COX-2.