| 硝普钠及其光解产物对日本晴水稻幼苗生长和5种激素标记基因表达的影响 |
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| 作者单位： | ;1.河南师范大学生命科学学院 |
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| 摘 要： | 以粳稻品种日本晴水稻为材料,检测硝普钠及其光解产物KNO_2、K_4Fe(CN)_6对幼苗生长的影响,并采用qRT-PCR技术检测了5种植物激素标记基因在经上述处理后在幼苗根中的表达水平.结果表明SNP能够显著抑制水稻幼苗的根长和株高;SNP对根生长的抑制主要是通过其光解产物K4Fe(CN)6实现的.SNP和K_4Fe(CN)_6处理都能够抑制生长素、细胞分裂素、脱落酸和赤霉素4种激素标志基因在水稻根中的表达,但是SNP处理可以抑制一氧化氮标志基因OsNOA1的表达,而K_4Fe(CN)_6则上调该基因的表达.
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| 关 键 词： | 硝普钠 水稻 幼苗 植物激素 基因表达 |
Effects of SNP and Its Photolysis Products on the Seedlings Growth of Rice and Expression of Marker Genes for Five Plant Hormones |
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| Institution: | ,College of Life Sciences,Henan Normal University |
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| Abstract: | Taking japonica cultivar Nipponbare rice as materials,the effects of sodium nitroprusside and its photolysis products KNO_2、K_4Fe(CN)_6 on the rice seedlings growth were analyzed,and marker genes for five different plant hormones in rice seedlings roots under the above treatments were detected by q RT-PCR in the study.The results showed that SNP can obviously inhibit the root length and plant height of the two rice seedlings,which implement mainly through its photolysis products K_4Fe(CN)_6.Moreover,the expression of the marker genes of auxin,cytokinins,abscisic acid and gibberellic acid were inhibited after the treatment of SNP and K_4Fe(CN)_6 in rice roots,but the marker gene of nitric oxide was inhibited by SNP,induced by K_4Fe(CN)_6. |
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| Keywords: | sodium nitroprusside rice seedling plant hormone gene expression |
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