丹参植株邻苯二甲酸与立枯丝核菌菌丝生长互作研究

为探讨导致丹参连作障碍的邻苯二甲酸与立枯丝核菌菌丝生长的相互作用，采用气相色谱法确定邻苯二甲酸的来源；通过室内菌丝生长抑制实验，研究邻苯二甲酸对立枯丝核菌菌丝生长的作用及临界浓度；采用高效液相色谱法测定菌丝生长抑制实验中培养体系在菌丝生长前后邻苯二甲酸含量的变化。结果显示：丹参植株地上部含有6种邻苯二甲酸酯类成分，总相对含量为8.264%；地下部含有5种邻苯二甲酸酯类成分，总相对含量为1.069%；邻苯二甲酸浓度对立枯丝核菌菌丝的生长存在低浓度促进，高浓度抑制的作用方式，在其浓度为0.1 mg/mL时，立枯丝核菌菌落数最多且菌落直径最大。在相同培养条件下，邻苯二甲酸的加入促进了立枯丝核菌生长繁殖，但其含量没有降低，证明邻苯二甲酸不是给立枯丝核菌生长提供碳源，而是起到相当于催化剂的作用。该研究旨在为克服丹参连作障碍提供新方法和理论依据。

Study on the interaction between phthalic acid and Rhizoctonia solanimycelium growth of Salvia miltiorrhiza Bge.

To investigate the continuous cropping barrier of Salvia miltiorrhiza Bge.caused by the interaction between phthalic acid and Rhizoctonia solani mycelium growth, the source of phthalic acid was determined by gas chromatography. The effect of phthalic acid on mycelia growth and its critical concentration were studied by laboratory mycelia growth inhibition experiment. The content of phthalic acid in the culture system before and after the growth of hyphae was determined using HPLC. The results showed six types of phthalate esters in the above ground part of S. miltiorrhiza, with a total relative content of 8.264%. The under ground part contains five types of phthalate esters, with a total relative content of 1.069%. Phthalic acid concentration promoted in low concentration and inhibited high concentration in the growth of R. solani. When the concentration was 0.1 mg/mL, the number of bacterial colonies of R. solani was the highest and the colony diameter was the largest. Under the same culture conditions, the addition of phthalic acid promoted the growth and reproduction of R. solani, but its content did not decrease, proving that phthalic acid did not provide C source for the growth of R. solani but acted as a catalyst. This study aimed to provide a new method and theoretical basis for overcoming the continuous cropping barrier of S. miltiorrhiza.