桦褐孔菌深层发酵培养条件的优化

目的优化桦褐孔菌深层发酵条件。方法采用L9(34)正交试验法对培养基进行优化。结果桑黄菌体发酵的最优方案是8%葡萄糖,1.60%酵母粉,0.3%KH2PO4,0.15%MgSO4,VB110mg/100mL,pH6.0,接种量为10%,装液量为100mL/300mL三角瓶,150r/min,恒温培养7天,生物量可达1.350g干菌丝每100mL发酵液。结论桦褐孔菌可以用深层液体发酵培养获得菌丝体,桦褐孔菌具有限氧生长的生物学特性。

Research on submerged fermentation process of phaeoporus obliquus

Aim To study the submerged fermentation process and amino acid analysis of Phaeoporus obliquus strain.Methods By adopting L 9(3 4) just cross experimentatiaon method to optimize culture medium.Results The composition of the optimal fermentation medium was as fellows:8%glucose,1.60%Powdery yeast,0.3%KH 2PO 4,0.15% MgSO 4,V B1 10mg/100mL,pH 6.0.The optimum cultural period was 7 day in 100/300 mL shake flasks at 150 r/min.The best aeration ratio was 2:5(medium volume:flask volume).The inoculation volume was 10%.The highest dry weight of mycelia of Phaeoporus obliquus was obtained about 1.350 g/150 mL in the optimum culture.Conclusion It is feasible that the mycelia of Phaeoporus obliquus were made by submerged fermentation process,Phaeoporus obliquus was provided with biological characteristic of limited oxygen.