| BYDV CP基因转化小麦原生质体及转基因植株再生 |
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| 引用本文： | 夏光敏,陈惠民,成卓敏,吴茂森. BYDV CP基因转化小麦原生质体及转基因植株再生[J]. 山东大学学报(理学版), 1996, 0(3) |
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| 作者姓名： | 夏光敏 陈惠民 成卓敏 吴茂森 |
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| 作者单位： | 中国农科院植保所 |
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| 摘 要： | 用原生质体融合技术与ＰＥＧ介导的直接转基因方法相结合，使高频率分裂的小麦济南１７７悬浮细胞系原生质体与具有分化能力的小麦济南１７７胚性意伤组织原生质体融合，形成具有分裂和分化能力的融合细胞；同时，利用ＰＥＧ对原生质体的直接转基因作用，将带有抗病毒基因（ＢＹＤＶＣＹｇｅｎｅ）的质粒Ｐｐｐ１５与带有抗性选择标记基因的质粒严ＰＢｌＡｃｔＳＮ导入融合细胞中．经抗性筛选获得抗潮霉素（Ｈｍ）的阳性克隆并再生植株．对再生植株作ＰＣＲ检测表明，ＣＰ基因已整合到转化植株的基因组中并稳定表达．
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| 关 键 词： | 小麦；原生质体融合；BYDVCP基因；共转化；转基因植株 |
TRANSFORMATION OF WHEAT PROTOPLASTS BY BYDV CP GENE AND THE REGENERATION OF TRANSGENIC PLANTS |
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| Xia Guangmin,Chen Huimin. TRANSFORMATION OF WHEAT PROTOPLASTS BY BYDV CP GENE AND THE REGENERATION OF TRANSGENIC PLANTS[J]. Journal of Shandong University, 1996, 0(3) |
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| Authors: | Xia Guangmin Chen Huimin |
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| Abstract: | The plasmid Ppp15 containing CP gene of BYDV and PBIActSN containing selectablemarker gene of hygromycin phosphotransperase for hygromycin (Hm) resistance wereused to cotransform the protoplasts of wheat (Tritioum aestivum L. )cv. Jinan 177.Two kinds of protoplasts were prepared. One of them originated from embryogeniccalli and were rarely capable of division in culture. The other were derived from noneregenerable cell suspension and able to divide with high frequency.They were mixed together with the two kinds of plasmid. Fusion and cotransformation were induced simultaneously by PEG method,and transgenic plants regeneratedafter hygromycin selection,which were confirmed by assay of PCR. |
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| Keywords: | wheat (Triticum aestivum L.) fusion of protoplasts BYDV CP gene cotransformation transgenic plants |
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