| 二肽衍生物的非水毛细管电泳分离 |
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| 引用本文： | 陈彦,王东芝,盛筱,刘素娟,索有瑞,尤进茂.二肽衍生物的非水毛细管电泳分离[J].曲阜师范大学学报,2007,33(3):81-84. |
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| 作者姓名： | 陈彦 王东芝 盛筱 刘素娟 索有瑞 尤进茂 |
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| 作者单位： | 曲阜师范大学化学科学学院 273165山东省曲阜市(陈彦，王东芝，盛筱，刘素娟，尤进茂)，中国科学院西北高原生物研究所 810001青海省西宁市(索有瑞) |
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| 摘 要： | 以1,2-苯并-3,4二氢咔唑-9-乙基氯甲酸酯(BCEOC)作为柱前衍生试剂,利用非水毛细管区带电泳法对衍生二肽进行分离,考察了该试剂用于非水毛细管区带电泳模式分离的几个关键条件,在有机溶剂甲醇与乙腈比例75∶25(V/V),醋酸铵浓度45 mmol/L,醋酸浓度0.8 mol/L,柱温17℃,分离电压28 kV条件下,采用二极管阵列检测,实现了11种二肽的基线分离.
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| 关 键 词： | 二肽 非水毛细管电泳 1 2-苯并-3 4二氢咔唑-9-乙基氯甲酸酯(BCEOC) |
| 文章编号： | 1001-5337（2007）03-0081-04 |
| 修稿时间： | 2006-11-30 |
Separation of Derivatized Dipeptides by Nonaqueous Capillary Electrophoresis |
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| CHEN Yan,WANG Dong-zhi,SHENG Xiao,LIU Su-juan,SUO You-rui,YOU Jin-mao.Separation of Derivatized Dipeptides by Nonaqueous Capillary Electrophoresis[J].Journal of Qufu Normal University(Natural Science),2007,33(3):81-84. |
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| Authors: | CHEN Yan WANG Dong-zhi SHENG Xiao LIU Su-juan SUO You-rui YOU Jin-mao |
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| Institution: | 1.College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong;2. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001,Xining,Qinghai, PRC |
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| Abstract: | A simple and rapid method for the separation of derivatized dipeptides,using 1,2-benzo-3-4dihydrocarbazole-9-ethyl chloraformate(BCEOC) as derivatization reagent by nonaqueous capillary electrophoresis is proposed for the first time.The effect of several key factors,such as proportion between MeOH and ACN,the concentrations of ammonium acetate and acetic acid,column temperature and voltage on separation,are studied respectively.Under the optimum experimental condition,a good baseline separation of 11 dipeptide derivatives is obtained. |
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| Keywords: | dipeptides nonaqueous capillary electrophoresis 1 2-benzo-3-4dihydrocarbazole-9-eth-yl chloraformate(BCEOC) |
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