荧光聚乙烯亚胺/曙红Y比率荧光法测定十二烷基硫酸钠

通过聚乙烯亚胺(PEI)与甲醛反应合成发蓝色荧光的聚乙烯亚胺(FPEI),根据十二烷基硫酸钠(SDS)与FPEI结合能力强于曙红Y(EY),使EY与FPEI间的距离增加,导致EY荧光恢复,并建立FPEI/EY双荧光体系测定SDS的比率荧光法.结果表明:SDS的浓度为0.62~60.00 μmol·L^-1,F₅₅₀/F₄₆₀与SDS的浓度具有较好的线性关系,检测限为0.062 μmol·L^-1,用于实际样品的测定,回收率

Detection of Sodium Dodecyl Sulfate by Ratiometric Fluorescence Based on Fluorescence Polyethyleneimine/Eosin Y

The fluorescent polyethyleneimine(FPEI)has been successfully synthesized by polyethyleneimine(PEI)reacted with formaldehyde, and FPEI exhibits strong blue emission. Since sodium dodecyl sulfate(SDS)has a stronger binding force with FPEI than EY, with the addition of SDS the distance between EY and FPEI became increased, which lead to the fluorescence of EY recovery. As such, a novel dual fluorescence system of FPEI/EY has been designed for ratiometric fluorescent detection of SDS. The study showed that F₅₅₀/F₄₆₀ of the FPEI/EY dual fluorescence system is linearly proportional to the concentration of SDS from 0.62 to 60.00 μmol ·L^-1 with a detection limit of 0.062 μmol ·L^-1. Real samples were analyzed with the recovery rate of 93.5%-105.5%.