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| 紫球藻多糖的分离纯化及抗菌活性 | |
| 引用本文： | 陈晓清,;郑怡,;林雄平,;许柑叶. 紫球藻多糖的分离纯化及抗菌活性[J]. 漳州师院学报, 2008, 0(2): 95-98 |
| 作者姓名： | 陈晓清, 郑怡, 林雄平, 许柑叶 |
| 作者单位： | [1]漳州师范学院生物科学与技术系,福建漳州363000; [2]福建师范大学生命科学学院,福建福州350108; [3]宁德高等师范专科学校生物系,福建宁德352100 |
| 基金项目： | 福建省自然科学基金资助项目（B0510008） |
| 摘 要： | 从紫球藻中提取的水溶性粗多糖，用Sevage法脱蛋白后，经DEAE-纤维素52柱层析可得到纯品．利用圆形纸片法对紫球藻多糖提取物进行了抑菌实验．结果表明，紫球藻多糖提取物对稻瘟病病原菌和赤皮病病原菌有较强的抑制作用，其抗细菌活性与抗真菌活性没有明显差别，对稻瘟病病原菌的最小抑菌浓度为6．25mg／mL． |
| 关 键 词： | 紫球藻 多糖 纯化 抗菌活性 |
Isolation,Purification and Antimicrobial Activities of the Polysaccharides from Porphyridium cruentum |
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| Affiliation: | CHEN Xiao-qing, ZHENG Yi, LIN Xiong-ping, XU Gan-ye (1.Department of Biology Science and Technology, Zhangzhou Normal University, Zhangzhou, Fujian 363000, China; 2.College of Life Sciences, Fujian Normal Univerity, Fuzhou, Fujian 350108, China; 3. Department of Biology, Ningde Teachers＇ College, Ningde, Fujian 352100, China) |
| Abstract: | The crude polysaccharides were extracted from Porphyridium cruentum by hot water .They were deproteinized by Sevage method for several times and purified by DEAE-cellulose. These crude extracts were tested the antibiotic activities using the agar disc diffusion method. The result showed that polysaccharides extracts from Porphyridium cruentum have strong activities against Pseudomonas fluorecens and Piricularia oryzae Cav. There were few differences between antibacterial activities and antifungal activities of polysaccharide extracts from Porphyridium cruentum. The minimum inhibition concentrations （MICs） of polysaccharides against Piricularia oryzae Cav were 6.25 mg/mL. |
| Keywords: | Porphyridium cruentum polysaccharide Purification antimicrobial activities |
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