大鼠颈总动脉球囊拉伤模型的制作研究

目的 通过对大鼠颈总动脉球囊拉伤模型制作技术的创新和探索,不断优化策略提高动物模型的成活率 和成材率,形成稳定的造模技术,为经皮冠状动脉腔内成形术术后并发症的预防和治疗提供实验基础。方法 将25只Sprague-Dawley（SD）大鼠随机分为三组,分别为大球囊组（球囊直径2.0 mm）11只、小球囊组（球囊直径 1.5 mm） 11只、假手术组3只,大球囊组和小球囊组应用不同大小的球囊进行模型制作,术后14 d通过伊凡氏蓝 染色进行验证,动脉拉伤后颈总动脉内膜成功着色者为造模成功。结果小球囊组造模成功10例（成功率 90.9%,10/11）,大球囊组造模成功5例（成功率45.5%,5/11）,两组造模成功率差异具有统计学意义,两组内膜 损伤面积无显著差异。结论 应用1. 5 mm球囊可以实现对内膜的有效损伤,且造模成功率较2. 0 mm球囊显著升高。

Establishment of Animal Model of Balloon Injury in Rat Carotid Artery

Objective To optimize the establishment of rat carotid artery balloon-injured model and to improve success rate in it. Method Twenty -five Sprague-Dawley ( SD) rats were randomly divided into three groups. The rats' left carotid artery in group A were deendothelialized by passing an inflatable balloon catheter ( size 2 mm, Cordis) . The rats' left carotid artery in group B were deendothelialized by passing an inflatable balloon catheter (size 1. 5 mm, Cordis) . The rats' left carotid artery in group C were made an arteriotomy without passing balloon catheter. The effect of balloon injury was assessed via Ivans ’ blue staining. Result The success rate in establishment of rat carotid artery balloon-injured model of group A is 45. 5% (5/11) . The success rate of group B is 90. 9% ( 10/11) ( P = 0. 032) . The difference of injured carotid artery intima area between group A and B is not significant ( P = 0. 426 ) . Conclusion The application of 1. 5 mm inflatable balloon catheter could successfully denude the intima of carotid artery in rats and could significantly improve the successful establish rate of rat carotid artery balloon-injured model.