红、蓝光照对大鼠视力早衰和神经网络的影响

目的探究红、蓝光照对大鼠视网膜衰老和神经网络的影响。方法将50只雄性Wistar大鼠随机分为5组,分别为蓝光照射1 h、蓝光照射8 h、红光照射1 h、红光照射8 h和空白对照组。干预12周后进行强制游泳实验(FSTs)评估精神状态。解剖取新鲜眼球视网膜色素上皮细胞(RPE)进行原代培养并进行衰老相关-β-半乳糖苷酶(SA-β-Gal)活性的鉴定,分析光照时间和复制衰老之间的关联,并采用试剂盒检测脑组织中一氧化氮(NO)和一氧化氮合酶(NOS)含量,分析光刺激对大鼠的应激反应。结果与红光照射相比较,蓝光光照8 h可显著上调RPE细胞的增殖(P<0.05)和NOS的含量(P<0.01),同时蓝光照射8 h更容易使大鼠陷入紧张、绝望的精神状态。结论蓝光较红光刺激对大鼠视网膜衰老和神经网络的影响更大。

Effects of Red and Blue Light on Premature Vision Failure and Neural Network in Rats

Objective To investigate the effects of red and blue light on retinal senescence and neural network in rats. Method Fifty male Wistar rats were randomly divided into 5 groups : blue light irradiation 1 h、blue light irradiation 8 h、red light irradiation 1 h,red light irradiation 8 h and blank control group. A compulsory swimming experiment ( FSTs) was performed after 12 weeks of intervention to assess mental state. The primary culture of fresh retinal pigment epithelial cells ( RPE) of the eyeball was dissected and senescence-associated-identification of β- galactosidase ( SA-β-Gal ) activity, analysis of the association between illumination time and replicative senescence, and use of reagents box to detect nitric oxide ( NO) and nitric oxide synthase ( NOS) content in brain tissue and analyze the stress response of light stimulation to rats. Result Compared with red light irradiation , blue light irradiation 8 h significantly up-regulated the proliferation of RPE cells (P <0. 05 ) and the content of ( P< 0.01 ) . At the same time, blue light irradiation 8 h more easily caused rats to fall into a nervous and desperate mental state. Conclusion The effect of blue light on retinal senescence and neural network is greater than that of red light.