hVEGFR2-KI小鼠血液生理生化参数的测定与分析

目的对不同性别的4周龄、8周龄C57BL/6-Kdrem1(h KDR)/NIFDC(以下简称hVEGFR2-KI)小鼠的血液参数进行测定,分析性别以及年龄对于该品系小鼠血液参数的影响。方法分别取20只4周龄、8周龄小鼠雌雄各半,采静脉血。用日本光电Mek7222血细胞分析仪测定hVEGFR2-KI小鼠的生理指标,奥林巴斯AU400全自动生化分析仪测定生化指标。结果在所测得的生理生化指标中,4周龄的hVEGFR2-KI小鼠雌雄之间于血红蛋白浓度(HGB)、总蛋白(TP)、总胆固醇(CHO)3项指标有显著差异(P<0.05),在红细胞计数(RBC)、红细胞压积(HCT)、平均红细胞血红蛋白(MCH)、平均红细胞血红蛋白浓度(MCHC)4项指标上有极显著差异(P<0.01)。8周龄的hVEGFR2-KI小鼠雌雄之间共有11项生理生化指标有极显著差异(P<0.01),分别是RBC、HCT、HGB、血小板计数(PLT)、血小板压积(PCT)、中性粒细胞绝对值(NEUT)、单核细胞百分率(MON%)7项血液生理指标和CHO、碱性磷酸酶(ALP)、葡萄糖(GLU)、甘油三酯(TG)4项血液生化指标;有5项具有显著差异(P<0.05)的指标,包括淋巴细胞百分率(LYM%)、中性粒细胞百分率(NEUT%)、TP、尿素(BUN)和磷(P)。不同周龄hVEGFR2-KI小鼠之间的差异分布更广,其中具有极显著差异(P<0.01)的有RBC、HCT、MCH、HGB、白细胞计数(WBC)、平均红细胞体积(MCV)、红细胞分布宽度(RDW)、平均血小板体积(MPV)、血小板分布宽度(PDW)、淋巴细胞绝对值(LYM)、单核细胞百分率(MON%)、TP、CHO、白蛋白(ALB)、ALP、肌酐(CREA)、GLU、P共18项指标;具有显著差异的为PLT、LYM%、NEUT%、丙氨酸氨基转移酶(ALT)、TG共5项指标。结论本次研究测定了不同性别的4周龄、8周龄的hVEGFR2-KI小鼠的生理生化指标,为hVEGFR2-KI小鼠的应用以及正常血液参数标准提供一定参考。

Determination and Analysis of Physiological and Biochemical Parameters of hVEGFR2-KI Mice

Objective To determine the blood parameters of hVEGFR2-KI mice at 4 weeks and 8 weeks of age of different genders, and to analyze the influence of gender and age on the blood parameters of this strain of mice. Method Venous blood was collected from 20 male and female mice aged 4 weeks and 8 weeks respectively. The physiological indexes of hVEGFR2-KI mice were determined by Japanese photoelectric Mek7 222 blood cell analyzer and Olympus AU400 automatic biochemical analyzer. Result Among the physiological and biochemical indexes measured, there was significant deference in hemoglobin concentration ( HGB ) , total protein ( TP) , total cholesterol ( CHO ) between male and female of 4-week-old hVEGFR2-KI mice ( P<0. 05 ) . There were significant differences( P<0. 01 ) in erythrocyte count ( RBC ) , hematocrit ( HCT ) , mean erythrocyte hemoglobin ( MCH ), mean erythrocyte hemoglob in concentration ( MCHC ) .There were significant differences in 11 physiological and biochemical indexes between male and female of 8 -week -old hVEGFR2-KI mice , which were blood physiological indexes including RBC , HCT ,HGB , platelet count , ( PLT ) , platelet volume , ( PCT ) , neutrophil absolute value (NEUT) , monocyte percentage ( MON%) and were 4 blood biochemical indexes such as CHO, alkaline phosphatase ( ALP) , glucose ( GLU) , TG; There are five statistically significant indicators, including percentage of lymphocytes ( LYM% ) , percentage of neutrophils ( NEUT% ) , TP , urea ( BUN ) and phosphorus( P ) . Differences of hVEGFR2-KI mice at different weeks of age were more widely distributed. They has a significant difference with RBC , HCT , MCH , HGB , white blood cell count ( WBC ) , average red blood cell volume ( MCV ) , red blood cell distribution width ( RDW) , mean platelet volume( MPV ) , platelet distribution width ( PDW) , lymphocyte absolute value ( LYM ) , monocyte percentage ( MON % ) , TP , CHO , albumin ( propagated ) , ALP , creatinine ( CREA) , GLU , P , a total of 18 indexes. PLT , LYM% , NEUT% , ALT and TG were the 5 indexes with statistical significance. Conclusion The physiological and biochemical indexes of hVEGFR2-KI mice aged 4 weeks and 8 weeks were determined in this study , which provided some reference for the application ofhVEGFR2-KImice and the standard of normal blood parameters.