遗传工程小鼠冻存卵巢异体原位移植比较研究 |
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引用本文: | 左 琴,王劲松,范 涛,朱婉月,刘秋菊,刘佐民.遗传工程小鼠冻存卵巢异体原位移植比较研究[J].实验动物科学,2020,37(3):65. |
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作者姓名: | 左 琴 王劲松 范 涛 朱婉月 刘秋菊 刘佐民 |
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摘 要: | 目的 对 C57BL / 6 J 为背景的遗传工程小鼠卵巢冷冻及复苏后原位移植进行研究,建立遗传工程小鼠卵巢
冷冻方法,弥补小鼠资源保种体系。 方法 采用 DAP213 方法冷冻保存了 C57BL / 6 J 及 SCARB2、PSGL1、IGB3S 三
个遗传工程小鼠 10 日龄幼鼠的卵巢,将四个品系 10 日龄幼鼠的新鲜卵巢及冻存复苏卵巢原位移植到 4 周龄
C57BL / 6 J 及 C57BL / 6 J( Cg) —Tyrc- 2 J / J( B6 albino)受体雌鼠,术后饲养 5 周与 10 周龄性成熟 C57BL / 6 J 雄鼠交
配,观察移植出生幼仔。 结果 C57BL / 6 J 新鲜卵巢和冻存卵巢,三个品系遗传工程小鼠的冻存卵巢原位移植到
C57BL / 6 J 和 B6 albino 受体中,均可以恢复正常功能,交配后得到仔鼠。 结论 采用 DAP213 方法冻存 C57BL / 6 J
及以 C57BL / 6 J 为背景的遗传工程小鼠卵巢,复苏后原位移植到 C57BL / 6 J 和 B6 albino,交配后产仔,卵巢冻存方
式是小鼠资源库保存的一个重要补充。
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关 键 词: | 原位移植 卵巢 冷冻保存 遗传工程小鼠 小鼠资源库 |
tudy for Cryopreservation and Orthotopic Transplantation
of Genetically Engineering Mice Ovary |
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Abstract: | Objective To explore a method of cryopreservation and orthotopic transplantation of genetically
engineering mice ovary of C57BL / 6 J background, establish a cryopreservation system. Method The ovaries from
10 d of C57BL / 6 J and three genetically engineering mice were freezed with DAP213 10 d fresh ovaries and thawed
ovaries were grafed to the 4 weeks C57BL / 6 J and C57BL / 6 J( Cg) —Tyrc- 2 J / J( B6 albino) recipient, then mated
with 10 weeks C57BL / 6 J male mice after 5 weeks breeding, observed offspring. Result The grafted C57BL / 6 J
fresh ovaries and freeze-thawed ovaries and genetically engineering mice freeze-thawed ovaries could be restored
reproduction, and gotten normal pups when mated with the same strain males. Conclusion Cryopreservation of
C57BL / 6 J and genetically engineering mice ovaries with DAP213, were thawed and grafted to recipient, then
mated with male, gotten normal pups. Cryopreservation of ovaries is a important method for mice resource bank. |
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Keywords: | orthotopic transplantation ovaries Cryopreservation Genetically engineering mice Mice resource bank |
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