| 人工培养条件下松材线虫提取物的毒性研究 |
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| 引用本文： | 曹越,沈伯葵. 人工培养条件下松材线虫提取物的毒性研究[J]. 南京林业大学学报(自然科学版), 1996, 39(4): 13. DOI: 10.3969/j.jssn.1000-2006.1996.04.003 |
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| 作者姓名： | 曹越 沈伯葵 |
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| 作者单位： | 南京林业大学森林资源与环境学院 |
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| 摘 要： | <正>将来源于黑松、马尾松的松材线虫分别用单异活体法和无异活体法人工培养后，以不同方法和不同溶剂对培养物进行提取，并用粗提浓缩液对３０ｄ以上生的黑松和马尾松幼苗做生物测定。结果表明，在人工培养条件下松材线虫无致萎毒素产生
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| 关 键 词： | 松材线虫；单异活体培养；无异活体培养；毒素；生物测定 |
STUDIES ON TOXICITY OF EXTRACTION OF PINE WOOD NEMATODES CULTIVATED IN ARTIFICIAL MEDIA |
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| Cao Yue Shen Bokui. STUDIES ON TOXICITY OF EXTRACTION OF PINE WOOD NEMATODES CULTIVATED IN ARTIFICIAL MEDIA[J]. Journal of Nanjing Forestry University(Natural Sciences ), 1996, 39(4): 13. DOI: 10.3969/j.jssn.1000-2006.1996.04.003 |
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| Authors: | Cao Yue Shen Bokui |
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| Affiliation: | College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037 |
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| Abstract: | After multiplication in monoxenic and axenic culture media, pine wood nematodes isolated from naturally infected Pinus thunbergii and P. massoniana are extracted individually in different ways and solvents. Nematode extracts are assayed by application of extracts to over 30-day-old seedlings of P.thunbergii and P. massoniana. It is clarified that wilt toxins are not produced by pine wood nematodes in artificial culture conditions. |
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| Keywords: | Pine wood nematodes Monoxenic culture Axenic culture Toxin Bioassay |
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