| 转基因鱼腥藻7120光异养生长的研究 |
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| 引用本文： | 成静,郭勇.转基因鱼腥藻7120光异养生长的研究[J].华南理工大学学报(自然科学版),2000,28(9):69-72. |
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| 作者姓名： | 成静 郭勇 |
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| 作者单位： | 华南理工大学食品与生物工程学院,广东广州 |
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| 基金项目： | 广东省自然科学基金资助项目!(980 541) |
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| 摘 要： | 研究了不同有机碳源对转基因鱼腥藻７１２０生长的影响，在此基础上进行了光异养条件的优化，并研究了外源添加剂Ｌ－精氨酸、生长激素２，４－Ｄ与ＫＴ对藻细胞生长的影响。结果表明：蔗糖和葡萄糖能促进藻体的生长；最优碳氮源组合为葡萄糖６ｇ／Ｌ、ＮａＮＯ３１．５ｇ／Ｌ，经高压灭菌；Ｌ－精氨酸不支持藻体的光异养生长；一定含量的２，４－Ｄ与ＫＴ能促进藻体在光异养条件下的后期生长。
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| 关 键 词： | 光异养生长 转基因鱼腥藻7120 基因工程 |
| 修稿时间： | 1999-10-14 |
Study on Photoheterotrophic Growth of Recombinant Anabaena sp. PCC7120 |
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| CHENG Jing,GUO Yong.Study on Photoheterotrophic Growth of Recombinant Anabaena sp. PCC7120[J].Journal of South China University of Technology(Natural Science Edition),2000,28(9):69-72. |
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| Authors: | CHENG Jing GUO Yong |
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| Abstract: | Studies on the effects of organic carbon sources as well as L_arginine and growth hormones 2,4_D and KT on the growth of recombinant Anabaena sp. PCC7120 were carried out. On the basis of research of carbon sources, the optimization of photoheterotrophic conditions were carried out too. The results show that saccharose and glucose can promote the growth of recombinant Anabaena sp. PCC7120. Optimum composition of carbon and nitrogen sources are 6 g/L glucose and 1.5 g/L NaNO 3 under high_pressure disinfection, that L_arginine does not support the photoheterotrophic growth of recombinant Anabaena sp. PCC7120, and that suitable content of 2,4_D and KT can stimulate the latter growth of recombinant Anabaena sp. PCC7120 under the photoheterotrophic conditions. |
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| Keywords: | Anabaena sp PCC7120 photoheterotrophic growth carbon source L_arginine growth hormone |
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