| 巨大芽孢杆菌AP25内切葡聚糖酶基因的克隆及序列测定 |
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| 引用本文： | 魏艳丽,周红姿,扈进冬,陈凯,黄玉杰,杨合同.巨大芽孢杆菌AP25内切葡聚糖酶基因的克隆及序列测定[J].山东科学,2009,22(5):22-26. |
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| 作者姓名： | 魏艳丽 周红姿 扈进冬 陈凯 黄玉杰 杨合同 |
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| 作者单位： | 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,山东济南250014 |
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| 摘 要： | 从土壤中分离到一株产纤维素酶的巨大芽孢杆菌AP25，经羧甲基纤维素平板检测，该菌可产生葡聚糖内切酶。根据GenBank登录的β-1，4内切葡聚糖酶基因（DQ782954．1，M28332．1，AY859492．1）的同源性序列，利用PCR方法克隆到该酶的基因，并对其进行测序。测序结果显示其全长为1500bp，推测其含499个氨基酸。与GenBank中的已知葡聚糖内切酶相比较，发现该酶的氨基酸序列与Bacillus subtilis的β-1，4内切葡聚糖酶基因同源性达到达94％。
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| 关 键 词： | 巨大芽孢杆菌 内切葡聚糖酶 基因克隆 序列分析 |
Cloning and Sequencing of Endo-Glucanase Gene of Bacillus.megaterium AP25 |
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| WEI Yan-li,ZHOU Hong-zi,HU Jin-dong,CHEN Kai,HUANG Yu-jie,YANG He-tong.Cloning and Sequencing of Endo-Glucanase Gene of Bacillus.megaterium AP25[J].Shandong Science,2009,22(5):22-26. |
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| Authors: | WEI Yan-li ZHOU Hong-zi HU Jin-dong CHEN Kai HUANG Yu-jie YANG He-tong |
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| Institution: | (Key Laboratory of Applied Microbiology of Shandong Province, Biotechnology Center of Shandong Academy of Sciences, Jinan 250014, China) |
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| Abstract: | Bacillus. megaterium AP25 screened from Australia wet soil could produce endo-β- glucanase that was detected on CMC-Na plates. We amplified the endo-β-1 ,4-glucanase gene sequenced it according to GenBank No. DQ782954.1, M28332.1, AY859492.1. Its full length 1500bp and its amino acid number was about 499. Its amino acid sequence had 98% homology that of the endo-β-1 ,4-glucanase gene of Bacillus subtilis. |
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| Keywords: | Bacillus megaterium endo-β-1 4-glucanase DNA cloning sequence analysis |
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