诺氟沙星与氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱

氧氟沙星(OFX)、诺氟沙星(NOR)皆能使牛血清白蛋白(BSA)的荧光猝灭.当2种药物共存时可进一步使BSA荧光猝灭,借此利用荧光光谱法研究了诺氟沙星、氧氟沙星药物间的相互作用.研究结果表明2种药物间存在相互作用,使药物与蛋白的结合稳定性减小;BSA的荧光猝灭属于静态猝灭,药物与BSA结合位点数n近似为1.依据F(o...

Studies on the Interaction Between Norfloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Fluorescence Spectrum Methods

Both ofloxacin(OFX) and norfloxacin(NOR) can quench fluorescence of bovine serum albumin(BSA).The fluorescence will quench further when two drugs coexist.According to this,studies on the interaction between NOR and OFX are established by fluorescence spectrum.Results showed that the interaction between the two drugs could reduce the binding stability of the protein and drug.It confirmed that the quenching mechanism of the combination between BSA and drugs was a static process,and the number of binding sites was 1.Based on the theory of Frester spectroscopy energy transfer,the binding distance(r<7.0 nm) between drugs and BSA was obtained.The correlation coefficient and binding distance were increased because of the existence of interaction between drugs.Studies utilizing synchronous spectra showed that the conjugation reaction between drugs and BSA would affect the conformation of BSA.It made protein molecules extend and hydrophobic nature reduce.