In vivo matrix-guided human mesenchymal stem cells |
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Authors: | J Kramer F Böhrnsen U Lindner P Behrens P Schlenke J Rohwedel |
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Institution: | (1) Department of Medical Molecular Biology, University of Lübeck, 23538 Lübeck, Germany;(2) Medical Department I, Division of Nephrology and Transplantation Unit, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany;(3) Institute of Immunology and Transfusion Medicine, University of Lübeck, 23538 Lübeck, Germany;(4) Department of Orthopaedic Surgery, University of Lübeck, 23538 Lübeck, Germany |
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Abstract: | Microfracture of subchondral bone results in intrinsic repair of cartilage defects. Stem or progenitor cells from bone marrow
have been proposed to be involved in this regenerative process. Here, we demonstrate for the first time that mesenchymal stem
(MS) cells can in fact be recovered from matrix material saturated with cells from bone marrow after microfracture. This also
introduces a new technique for MS cell isolation during arthroscopic treatment. MS cells were phenotyped using specific cell
surface antibodies. Differentiation of the MS cells into the adipogenic, chondrogenic and osteogenic lineage could be demonstrated
by cultivation of MS cells as a monolayer, as micromass bodies or mesenchymal microspheres. This study demonstrates that MS
cells can be attracted to a cartilage defect by guidance of a collagenous matrix after perforating subchondral bone. Protocols
for application of MS cells in restoration of cartilage tissue include an initial invasive biopsy to obtain the MS cells and
time-wasting in vitro proliferation and possibly differentiation of the cells before implantation. The new technique already includes attraction
of MS cells to sites of cartilage defects and therefore may overcome the necessity of in vitro proliferation and differentiation of MS cells prior to transplantation.
Received 3 November 2005; received after revision 15 December 2005; accepted 4 January 2006 |
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Keywords: | Mesenchymal stem cells cartilage AMIC extracellular matrix chondrocytes adipocytes osteocytes |
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