| 药用植物桔梗组培快繁体系的建立 |
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| 引用本文： | 唐伟斌,石晓云.药用植物桔梗组培快繁体系的建立[J].河北师范大学学报(自然科学版),2006,30(5):599-601. |
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| 作者姓名： | 唐伟斌 石晓云 |
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| 作者单位： | 邢台学院,生物系,河北,邢台,054001;邢台学院,生物系,河北,邢台,054001 |
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| 基金项目： | 河北省科技厅科技攻关项目
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邢台学院校科研和教改项目 |
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| 摘 要： | 以药用植物(桔梗)种发无菌苗幼嫩茎尖、茎段为外植体,附加不同种类、不同质量浓度的激素进行组织培养,建立了快速繁殖体系.得出如下最佳培养基组合:诱导分化培养基MS 6-BA1.0mg/L NAA0.05 mg/L;增殖培养基MS 6-BA0.4 mg/L NAA 0.1 mg/L;生根培养基1/2 MS NAA0.5 mg/L和1/2MS IBA0.5 mg/L.为桔梗优良株系的繁育和利用提供了有效途径.
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| 关 键 词： | 桔梗 快速繁殖 诱导 愈伤组织 |
| 文章编号： | 1000-5854(2006)05-0599-03 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005-12-19 |
Establishment of Tissue Culture and Rapid Proliferation System for Medical Plant Platycodon grandiflorum |
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| TANG Wei-bin,SHI Xiao-yun.Establishment of Tissue Culture and Rapid Proliferation System for Medical Plant Platycodon grandiflorum[J].Journal of Hebei Normal University,2006,30(5):599-601. |
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| Authors: | TANG Wei-bin SHI Xiao-yun |
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| Institution: | Department of Biology,Xingtai College,Hebei Xingtai 054001,China |
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| Abstract: | To establish the rapid proliferation system of the medical plant Platycodon grandiflorum.The shoot apices and segment of P. grandiflorum were used as explants and cultured on different media supplemented with various portion of phytohormone, MS medium containing 6-BA 1.0 mg/L and NAA 0.05 mg/L. was the best for callus induction;MS medium containing 6-BA 0.4 mg/L. and NAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IBA 0.5 mg/L. and 1/2 MS medium containing NAA 0. 5 mg/L, were suitable for inducing root. The rapid proliferation of P. grandiflorum by tissue culture method could be established, which will provide the effective ways of the breeding and clone of excellent varieties and utilization of P. grandiflorum. |
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| Keywords: | Platycodon grandiflorum rapid proliferation induction callus |
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