Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice (Oryza sativa L.) |
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Authors: | Ziyin Li Shouyi Chen |
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Institution: | (1) Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciencs, 100101 Beijing, China |
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Abstract: | Resistance-like sequences have been amplified from first strand cDNA and genomic DNA of rice by PCR using oligonucleotide
primers designed from sequence motifs conserved between resistance genes of tobacco andArabidopsis thaliana. 3 PCR clones, designatedOsr1, Osr2 andOsr3 which were 98% identical in nucleotide sequence level, have been found to be significantly homologous to known plant resistance
genes and all contained the conserved motifs of NBS-LRR type resistance genes, such as P-loop, kinase2a, kinase3a and transmembrane
domain.Southern hybridization revealed that rice resistance gene hornologueswere organized as a cluster in the genome. RFLP mapping using a DH population derived from anindica/japonka cross (Zhaiyeqing 8/Jingxi 17) and an RFLP linkage map assigned two copies ofOsrl and one copy ofOsr3 to the distal position of chromosome 12 where a blast resistance QTL has been mapped previously. Northern blot analysis showed
thatOsrl gene was constitutively transcribed in rice leaves, shoots and roots. Further study concerning isolation of full-length cDNAs
would be conducive to elucidating the functions of these genes. |
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Keywords: | rice resistance gene homologue rice blast gene expression |
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