| 猪心肌细胞色素C两种纯度测定法的比较和结晶的制备 |
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| 引用本文： | 王怀栋,张光兴.猪心肌细胞色素C两种纯度测定法的比较和结晶的制备[J].河北大学学报(自然科学版),1986(1). |
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| 作者姓名： | 王怀栋 张光兴 |
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| 作者单位： | 河北大学生物系
(王怀栋)，河北大学生物系(张光兴) |
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| 摘 要： | 本文介绍了用纯化的猪心肌细胞色素C—与溶菌酶掺和的样品,比较其百分含铁量法和E_(280)~(c2+)/E_(280)~(c3+)比值法两种不同的纯度测定方法,证明二者有很好的线性关系。光谱比值法与百分铁量法比较,有需要样品少,操作简便、迅速等优点。 此外还报导了经纯化和浓缩的猪心肌细胞色素C用饱和硫铵制备出结晶的简便方
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Comparison of Two Determined-Purety Methods and Crystallized Procedure For Pig Heart Muscle Cylochrotne C |
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| Abstract: | In this paper, two detcrmined-purety methods (fcrro-porc-ent & E550c2+/E280c3+ Spectrum ratios) have been compared, with
mixed samples of purified pig heart muscle cytochrome C and lysozyme in different percent. The results show that a better linear correlation has been maintained between ferro-percerit
and E550c2+/E280c3+ spectrum ratios. And spectrum ratios is better
than ferro-pcrcent at using little sample, simple procedure and fast speed determination. |
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