模拟失重效应下龙血素B在大鼠体内的药物动力学及排泄

比较正常重力与模拟失重效应下大鼠口服龙血素B的血浆药物动力学及排泄差异.大鼠吊尾21 d模拟失重效应，单次灌胃给予25 mg/kg龙血素B.于系列时间点收集静脉血，并分时间段收集尿液、粪便和胆汁，采用HPLC-MS方法测定各样本中龙血素B含量，并求算药物动力学参数和排泄量.模拟失重效应下龙血素B的血药浓度-时间曲线下面积（AUC_0-t）、血药峰浓度（C_max）、达峰时间（t_max）、表观分布容积（V_d）、消除速率常数（K_e）以及生物半衰期（T_1/2）与正常重力组相比均出现显著变化，说明模拟失重效应明显改变龙血素B在大鼠体内的药物动力学过程.尿液、粪便和胆汁中龙血素B的排泄量也在增加，但与正常重力相比不明显. 

Study on Pharmacokinetics and Excretion of Loureirin B in Rats Under Simulated Weightlessness Condition

To study pharmacokinetics and excretion difference of loureirin B (LB) in rats after single oral administration between simulated weightlessness and gravitation condition, a series of test was executed. Rats were tail-suspended for 21 days to simulate weightlessness effect. After a single oral administration of 25 mg/kg LB, venous blood and excretion samples were collected at different time points. Concentration of LB in samples was determined by HPLC-MS method. Pharmacokinetic parameters and excretion amount were calculated. Results show that, compared with normal gravity rats, the area under cuve (AUC_0-t), maximum concentration (C_max), time to peak concentration (t_max), volume of distribution (V_d), elimination rate constant (K_e), and biological half-life (T_1/2) of simulated weightless rats ware significantly changed. Meanwhile, the excretion of LB in urine, feces, and bile are also up-regulated in simulated weightlessness group, while no obvious differences are observed.