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| PCR检测白血病患者外周血中EB病毒的DNA | |
| 引用本文： | 卢育洪,周羽竝,杨力建,陈少华,李扬秋.PCR检测白血病患者外周血中EB病毒的DNA[J].暨南大学学报,2000,21(4):100-103. |
| 作者姓名： | 卢育洪 周羽竝 杨力建 陈少华 李扬秋 |
| 作者单位： | 1. 暨南大学医学院,第一附属医院血液科,广东,广州,510630 2. 暨南大学医学院,生化教研室,广东,广州,510632 3. 暨南大学医学院,血液病研究室,广东,广州,510632 |
| 摘 要： | 目的：了解白血病患者ＥＢ病毒感染情况。方法：收集２１例急性淋巴细胞白血病、１例慢性淋巴细胞白血病、１５例急性粒细胞白血病、８例慢性粒细胞白血病患者及３２例正常对照组的外周血，分离单个核细胞，提取ＤＮＡ，应用ＰＣＲ方法检测ＥＢ病毒ＤＮＡ。结果：在１例初诊慢性粒细胞白血病病人样本中发现ＥＢ病毒阳性，余均为阴性。结论：白血病患者存在ＥＢ病毒感染情况，但并不普遍。 |
| 关 键 词： | 白血病 EB病毒 聚合酶链反应 检测 DNA PCR方法 |
| 文章编号： | 1000-9965(2000)04-0100-04 |
| 修稿时间： | 1999-12-28 |
Detection of Epstein-Barr virus DNA in leukemia patients by polymerase chain reaction |
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| LU Yu-hong,ZHOU Yu-bing,YANG Li-jian,CHEN Shao-hua,LI Yang-qiu.Detection of Epstein-Barr virus DNA in leukemia patients by polymerase chain reaction[J].Journal of Jinan University(Natural Science & Medicine Edition),2000,21(4):100-103. | |
| Authors: | LU Yu-hong ZHOU Yu-bing YANG Li-jian CHEN Shao-hua LI Yang-qiu |
| Institution: | LU Yu-hong ;(Dept.of Internal Medicine,First Affiliated Hospital of JNUMC,Guangzhou 510630,China);ZHOU Yu-bing ;(Dept.of Biochemistry,Guangzhou 510632,China);YANG Li-jian ,CHEN Shao-hua ,LI Yang-qiu ;(Dept.of Hematology,JNUMC,Guangzhou 510632,China) |
| Abstract: | Aim: To detect Epstein-Barr virus DNA in the leukemia patients. Methods: To collect perpherial blood from patients of 21 cases with acute lymphoblastic leukemia(ALL); One case with chronic lymphocyte leukemia(CLL);fifteen cases with acute myeloid leukemia(AML);eight cases with chronic myelogenous leukemia(CML) ,and from 32 normal control people. DNA is extracted from perpherial blood mononuclear cells and EBV-DNA is delected by PCR. Results: BEV -DNA was detected in a case with CML. Conclusion: EBV infections could be found in leukemia patients , but not general. |
| Keywords: | PCR Epstein-Barr virus leukemia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |