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| Apoptosis Induced by High Concentrations of Nicotinamide in Tobacco Suspension Cells | |
| 引用本文： | 张贵友 朱瑞宇 戴尧仁. Apoptosis Induced by High Concentrations of Nicotinamide in Tobacco Suspension Cells[J]. 清华大学学报, 2004, 9(1): 88-93 |
| 作者姓名： | 张贵友 朱瑞宇 戴尧仁 |
| 作者单位： | DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China |
| 基金项目： | the National Natural Science Foundation of China (No. 39970072) |
| 摘 要： | As an inhibitor of poly(ADP-ribose) polymerase (PARP), nicotinamide has a restraining effect on apoptosis at certain low concentrations. In our present study, apoptosis induced by high concentrations of nicotinamide was observed in tobacco suspension cells. When cells were preincubated with 250 mmol/L nicotinamide for 24 h, the hallmarks of apoptosis were detected, including DNA fragments increasing in size by multiples of 180-200 bp，the condensation and peripheral distribution of nuclear chromatin, and a positive reaction to the TUNEL assay. At the same time, the degradation of PARP and the reduction in the potential of the inner membrane of mitochondria appeared in apoptotic cells induced by high concentrations of nicotinamide. This result indicates that apoptosis induced by high concentrations of nicotinamide is associated with caspase-3-1ike activity and with the opening of mitochondrial permeability pores. These results partially support the hypothesis that high concentrations of PARP inhibitor could force cells to enter an apoptotic pathway by delay of DNA repair in replicating cells. |
| 关 键 词： | 细胞凋亡 烟碱 烟草 磷脂酰丝氨酸 |
Apoptosis Induced by High Concentrations of Nicotinamide in Tobacco Suspension Cells |
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| ZHANG Guiyou ,ZHU Ruiyu,DAI Yaoren. Apoptosis Induced by High Concentrations of Nicotinamide in Tobacco Suspension Cells[J]. Tsinghua Science and Technology, 2004, 9(1): 88-93 | |
| Authors: | ZHANG Guiyou ZHU Ruiyu DAI Yaoren |
| Affiliation: | ZHANG Guiyou **,ZHU Ruiyu,DAI Yaoren Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
| Abstract: | As an inhibitor of poly(ADP-ribose) polymerase (PARP), nicotinamide has a restraining effect on apoptosis at certain low concentrations. In our present study, apoptosis induced by high concentrations of nicotinamide was observed in tobacco suspension cells. When cells were preincubated with 250 mmol/L nicotinamide for 24 h, the hallmarks of apoptosis were detected, including DNA fragments increasing in size by multiples of 180-200 bp, the condensation and peripheral distribution of nuclear chromatin, and a positive reaction to the TUNEL assay. At the same time, the degradation of PARP and the reduction in the potential of the inner membrane of mitochondria appeared in apoptotic cells induced by high concentrations of nicotinamide. This result indicates that apoptosis induced by high concentrations of nicotinamide is associated with caspase-3-like activity and with the opening of mitochondrial permeability pores. These results partially support the hypothesis that high concentrations of PARP inhibitor could force cells to enter an apoptotic pathway by delay of DNA repair in replicating cells. |
| Keywords: | apoptosis nicotinamide tobacco suspension cell poly (ADP-ribose) polymerase |
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