三维大孔氮化碳材料的制备及其血液相容性

以粒径640 nm的单分散二氧化硅胶体晶体为模板,由四氯化碳和乙二胺回流加热制备出氮化碳的前驱物;将其填入模板的缝隙中,在氮气中热处理,形成氮化碳/二氧化硅的复合物;用氢氟酸除去二氧化硅模板,制备出三维大孔氮化碳材料. 通过扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射 (XRD)、选区电子衍射(SAED)、元素分析、红外光谱(FT-IR)、X射线光电子能谱(XPS),对其形貌结构、元素组成、键合状态进行了形貌和结构的表征. 采用部分凝血活酶时间(APTT)、凝血酶原时间(PT)和凝血酶时间(TT)对其体外抗凝血活性作了初步的评价,发现制备的大孔氮化碳对血液不会造成促凝,说明其可能成为一种新的血液相容性材料.

Synthesis of 3D macroporous carbon nitride and its blood compatibility

Monodisperse silica spheres with a diameter of 640nm were used as template, carbon nitride precursor was made by refluxing and stirring ethylendiamine (CH_2NH_2)_2 and carbon tetrachloride (CCl_4). The precursor was infiltrated into the interstitial regions of the silica template and heat-treated in nitrogen atmosphere to form a carbon nitride/silica composite, then macroporous carbon nitride was gained by removing the silica template with HF solution treatment. The composition and structure of a macroporous carbon nitride sample was detected by X-ray powder diffraction (XRD), elemental analysis, selected area electron diffraction (SAED), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectra (XPS). The in vitro anticoagulation activity of the sample was evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT). It is found that the macroporous carbon nitride has no influence on the anticoagulation activity of plasma. It indicates that the potential application of the 3D macroporous carbon nitride as blood compatibility materials in the biomedical area will be realized in the future.