| 豆凝乳酶产生菌的筛选及诱变育种 |
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| 引用本文： | 蒋咏梅,章文贤,施巧琴,吴松刚.豆凝乳酶产生菌的筛选及诱变育种[J].福建师范大学学报(自然科学版),2000,16(1):89-93. |
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| 作者姓名： | 蒋咏梅 章文贤 施巧琴 吴松刚 |
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| 作者单位： | 福建师范大学生物工程学院,福建,福州,350007 |
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| 摘 要： | 从１０４份土样中分离到一株产豆凝乳酶的芽孢杆菌（Ｂａｃｉｌｌｕｓ ｓｐ．），酶活力０．３ｕ／ｍｌ。经ＵＶ－ＤＥＳ诱变及培养基调整后，酶活可达１．６ｕ／ｍｌ以上，其最适产酶培养基组成：麦麸２．５％，（ＮＨ４）２ＳＯ４ ０．５％（ＮＨ４）２ＨＰＯ４ ０．５％，ＣａＣｌ２ ０．０５％。
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| 关 键 词： | 筛选 芽孢杆菌 豆凝乳酶产生菌 分离 诱变育种 |
Study on Soymilk-clotting Enzyme Producing Bacteria |
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| JIANG Yong-mei,ZHANG Wen-xian,SHI Qiao-qin,WU Song-gang.Study on Soymilk-clotting Enzyme Producing Bacteria[J].Journal of Fujian Teachers University(Natural Science),2000,16(1):89-93. |
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| Authors: | JIANG Yong-mei ZHANG Wen-xian SHI Qiao-qin WU Song-gang |
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| Abstract: | A strain of Bacillus producing soymilk clotting enzyme was isolated from 104 soil samples. After mutagenic treatment and fermentation test, its soymilk clotting enzyme activity could reach 1 6u/ml. The optimum medium consisted of 2 5% wheat bran, 0 5% (NH 4) 2SO 4 , 0 5% (NH 4) 2HPO 4 and 0 05% CaCl 2. |
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| Keywords: | soymilk clotting enzyme screening mutagenesis Bacillus |
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