| 氰戊菊酯对乳腺癌细胞MCF-7增殖的影响 |
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| 引用本文： | 赵波,郎朗,季宇彬.氰戊菊酯对乳腺癌细胞MCF-7增殖的影响[J].哈尔滨商业大学学报(自然科学版),2012(2):129-132,139. |
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| 作者姓名： | 赵波 郎朗 季宇彬 |
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| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心;国家教育部抗肿瘤天然药物工程研究中心 |
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| 基金项目： | 黑龙江省骨干教师项目(1155G23) |
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| 摘 要： | 通过加入PD98059(细胞外信号调节激酶阻断剂)后探讨氰戊菊酯诱导乳腺癌细胞增殖的作用机制.观察加入PD98059前后氰戊菊酯诱导的细胞增殖情况,采用MTT法,流式细胞仪测定周期法,流式细胞仪法观察ERα蛋白、ERK蛋白和p-ERK1/2蛋白表达情况.氰戊菊酯能够明显升高MCF-7细胞的S期细胞数,作用程度与雌二醇相似,并且通过降低ERα蛋白表达量,升高p-ERK蛋白表达量促进MCF-7细胞的增殖;加入PD98059后,S期细胞比例降低,ERα蛋白表达量升高,p-ERK蛋白表达量降低,MCF-7细胞的增殖降低.氰戊菊酯通过ERK细胞信号转导通路发挥促细胞增殖作用.
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| 关 键 词： | 氰戊菊酯 PD98059 MCF-7细胞 ERα ERK1/2 p-ERK1/2 |
Effect of fenvalerate on proliferation of breast cancer cell line MCF-7 |
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| ZHAO Bo,LANG lang,JI Yu-bin.Effect of fenvalerate on proliferation of breast cancer cell line MCF-7[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2012(2):129-132,139. |
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| Authors: | ZHAO Bo LANG lang JI Yu-bin |
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| Institution: | 1,2(1.Harbin University of Commerce,Research Center on Life Science and Environmental Science,Harbin 150076,China; 2.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China) |
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| Abstract: | To explore mechanism of effect of fenvalerate on the proliferation of breast cancer cell line MCF-7 by adding PD98059(extracellular signal regulating kinase blockers).MTT assay was used to detect the proliferation of MCF-7.Cell cycle of MCF-7 and the expression of ER α,ERK,p-ERK1/2 were evaluated by flow cytometry.The result showed that fenvalerate was similar with estradiol,could increase the MCF-7 cells in S phase.Additionally,the fenvalerate prompt the proliferation of MCF-7 by down-regulating the expression of ER α and up-regulating p-ERK.Contrary to fenvalerate,reduction of MCF-7 cell proliferation,the up-regulated ERα and down-regulated p-ERK were due to PD98059.The results showed that fenvalerate prompted the proliferation of MCF-7 by ERK signal transduction pathways. |
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| Keywords: | fenvalerate PD98059 MCF-7 cells ERα ERK1/2 p-ERK1/2 |
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