MBR+蠕虫床耦合系统内胞外聚合物三维荧光特性

采用三维荧光技术研究了MBR+蠕虫床耦合系统内蠕虫作用对MBR内胞外聚合物(EPSS及EPSB)特性的影响.MBR-A、MBR-B和SSBWR内EPSS的EEM光谱中均主要有四个特征峰,SS-BWR的作用未破坏MBR-B内EPSS中酪氨酸类蛋白质的结构,使色氨酸类蛋白质的峰发生了较大程度的红移,两类芳香性蛋白质、类腐殖酸以及类富里酸物质含量均有大量增加.耦合系统内EPSB的EEM光谱均主要有两个特征峰,分别为酪氨酸和色氨酸类蛋白质,EPSB主要由蛋白质类物质构成.经SSBWR作用后EPSB中两类蛋白质含量减少明显,并且色氨酸类蛋白质的特征峰产生了5～10nm的蓝移.MBR-B的污泥混合液经SSBWR作用后,其I(B)/I(A)值有了一定程度的降低,蠕虫的捕食作用使污泥混合液中易生物降解的有机质减少,降低了污泥混合液的可生化性.

Study on characterization of extracellular polymers in MBR-sessile worm reactor system by using three-dimensional excitation and emission matrix fluorescence spectroscopy

Fluorescence excitation emission matrix spectroscopy(EEMS)was used to study the impact of worms on the extracellular polymers(EPSS and EPSB) in MBR-sessile worm reactor system.There were four peaks in MBR-A,MBR-B and SSBWR.SSBWR did not destroy the tyrosine’s structure of EPSS in MBR-B,however,lead to a large read shift of tryptophan.Those two kinds of protein,fulvic acid-like and humic acid-like increased greatly in quantity.Two peaks were found in the EEM of EPSB in the combined system,which was tyrosine and tryptophan.So,EPSB was consisted of substances like protein.SSBWR lead to a significant decrease in the quantity of those two kinds of protein,in addition,result in a 5~10 nm blue shift in the peak of tryptophan.After sludge mixed liquor in MBR-B was predated by worms in SSBWR,I(B)/I(A) decreased at a certain degree.The amount of organic matter which was easily degraded in sludge mixed liquor and the biodegraded ability droped,due to the predation of worms.