人工感染呼肠孤病毒对拟穴青蟹部分免疫因子的影响

采用注射和浸泡的方式人工感染拟穴青蟹呼肠孤病毒,研究了其对拟穴青蟹血细胞密度以及血清中酚氧化酶(PO)、超氧化物歧化酶(SOD)和碱性磷酸酶(AKP)活力的影响.结果表明:注射(第Ⅱ组)和浸泡(第Ⅳ组)方式均能感染健康青蟹,病发死亡时间为7～9 d,死亡率达100%,血细胞平均密度在试验36～72 h间迅速上升并达最高值,其血细胞密度最高值分别为1.4008×107cells/mL与1.8243×107cells/mL;感染病毒的青蟹PO活性均大致呈现下降的趋势,其中,第Ⅳ组感染12 h青蟹PO活性相对对照组显著升高(P<0.05),其值为(6.90±1.54),达实验所测PO活性最高值;各实验组血清SOD活性呈无规律的变化;AKP活性感染组与对照组表现不同.表明测定血细胞密度以及PO和AKP活性可用来辅助诊断青蟹疾病,而SOD活性变化不能很好地表征青蟹受病毒感染的状况.

Effects of Artifical Infection Reovirus Trails on Some Immune Factors of Mud Crab,Scylla paramamosain

The influence of artifical infection reovirous trails on the hemocyte density and the phenoloxidase(PO),superoxide dismutase(SOD) and alkaline phosphatase activity(AKP) of the serum from Mud Crab,Scylla paramamosain was examined.The results showed that the healthy crab would be infected by virous injection and bath exposure in the virus extraction,There were no obvious difference between two treatments in mortality and latency period of the experimental animals,all crabs died in 7~9 d after infection.Changes of some interrelated immune factors such as activities of PO,SOD and AKP displayed the downtrend day by day,while the hemocyte density rose evidently after 36 h and reached the peak value(2.8534×107 cells/mL) at 72 h,It recovered to the same level as the control group after 108 h.In conclusion,the changes of hemocyte density and PO,AKP activity except SOD activity would be used to assist diagnoses of crab diseases.